Purchase this article with an account.
Shinichiro Kobayakawa, Toru Matsunaga; The Preventive Effect by the Drug Released Soft Contact Lens against Bacterial Endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2923.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
We developed a drug released soft contact lens (DR-SCL) of a hydrogel material that releases antibiotics in a sustained manner. The purpose of this study was to investigate the preventive effect of the DR-SCL to bacterial endophthalmitis in a rabbit model.
Gatifloxacin (GFLX) 0.3% eye drops were used. Staphylococcus aureus (MRSA, ATCC 43300) was used to induce experimental endophthalmitis. Each DR-SCL was soaked in GFLX 0.3% eye drop solutions and uptake of the antibiotic using an ion ligand mechanism. Forty-five Japanese albino rabbits were divided into three groups post-inoculation of S.aureus to anterior chamber. DR-SCL group, SCLs presoaked with GFLX were administered to eyes (n=15); control group, SCLs without antibiotics administered to eyes (n=15); eye drop group, topical GFLX was administered to eyes twice or three times every four hours for three days (n=15). At 24, 48, and 72 hr post-inoculation, five rabbits from each group were euthanized, and those inoculation eyes enucleated for the enumeration of bacteria. Moreover, those eyes were evaluated by slit-lamp biomicroscopy examination at each time-point.
All rabbits in the control group developed severe intraocular infection. The bacterial populations were significantly smaller in DR-SCL (0 log10CFU/ml) and eye drops group (3.54-4.17 log10CFU/ml) than in the control group (4.82-7.3 log10CFU/ml) through 72 hours after inoculation (p<0.01). Most importantly, bacterial populations were undetectable in the DR-SCL group throughout the experimental periods. The DR-SCL group populations were significantly smaller than those in the eye drop group at 24 hours. However, there were no significant differences at 48 hours and in the eye drop group, and the populations were undetectable at 72 hours. Inflammation scores of the DR-SCL group were significantly smaller than of the eye drops group, and of the control group (p<0.05) at 48 and 72 hours.
DR-SCL contained with GFLX prevented to the growth of S.aureus through 72 hours. It was suggested that DR-SCL have a preventive effect against acute postoperative endophthalmitis.
This PDF is available to Subscribers Only