June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
The effect of total lens epithelial cell eradication on intraocular lens stability in the human capsular bag
Author Affiliations & Notes
  • Julie Eldred
    School of Biological sciences, University of East Anglia, Norwich, United Kingdom
  • Sarah Russell
    School of Biological sciences, University of East Anglia, Norwich, United Kingdom
  • Richard Evans-Gowing
    School of Biological sciences, University of East Anglia, Norwich, United Kingdom
  • Michael Wormstone
    School of Biological sciences, University of East Anglia, Norwich, United Kingdom
  • David Spalton
    The Consulting Rooms, King Edward VII's Hospital, London, United Kingdom
  • Footnotes
    Commercial Relationships Julie Eldred, None; Sarah Russell, None; Richard Evans-Gowing, None; Michael Wormstone, University of East Anglia (P), Alcon Laboratories (C), AnewOptics (C); David Spalton, B and L (C), Santen/AVS (C), AnewOptic (I)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2975. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Julie Eldred, Sarah Russell, Richard Evans-Gowing, Michael Wormstone, David Spalton; The effect of total lens epithelial cell eradication on intraocular lens stability in the human capsular bag. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2975. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Complete removal of lens epithelial cells (LEC) is a strategy to prevent posterior capsule opacification, the major complication of cataract surgery. Intraocular lens (IOL) stability within the capsular bag is important to good visual outcome. We therefore investigated how total LEC loss affects IOL stability within the capsular bag.

Methods: Capsular bags were generated from human donor eyes by capsulorhexis and lens extraction followed by implantation of a single piece Acrysof IOL. Capsular bags with associated zonules and ciliary body were removed from the eye and secured by pinning the ciliary body to a silicone ring. Capsular bags were maintained in 6ml EMEM supplemented with 5%FCS and 10ng/ml TGFβ2 for ≥ 3 weeks. One bag of each pair was treated with 1µM thapsigargin, a calcium ATPase inhibitor; this was employed to destroy all LECs. Observations of LEC growth were captured by phase contrast microscopy. IOL stability within the bag was assessed by video microscopy. At end-point the bags were examined using scanning electron microscopy (SEM) and immunocytochemistry.

Results: LECs in control capsular bags could be observed to migrate centrally closing the bag and fixating the IOL between the anterior and posterior capsules, as seen clinically. In addition a firm seal was formed at the rhexis edge between the anterior capsule and the underlying IOL. Application of thapsigargin to the capsular bags prevented cell growth and led to a complete loss of viable cells. Consequently, thapsigargin treated preparations did not exhibit adhesion between anterior and posterior capsules nor adhesion to the IOL surface. At end point these observations were confirmed by SEM and immunocytochemistry. Following a period of controlled orbital movement, the positioning within the capsular bag was unaffected in each test group. However, the IOL in the control group stabilized quicker than in the thapsigargin treated bags.

Conclusions: LECs appear to aid stabilization of current IOL designs in the capsular bag. This study has important clinical implications for IOL design and for strategies to prevent posterior capsule opacification.

Keywords: 445 cataract • 567 intraocular lens • 652 posterior capsular opacification (PCO)  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×