June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
PTF1a in anterior eye development in zebrafish
Author Affiliations & Notes
  • C Denwood
    Ophthalmology, Indiana University Glick Eye Institute, Indianapolis, IN
  • Nisha Kheradiya
    Ophthalmology, Indiana University Glick Eye Institute, Indianapolis, IN
  • Na Luo
    Ophthalmology, Indiana University Glick Eye Institute, Indianapolis, IN
  • Michael Conwell
    Ophthalmology, Indiana University Glick Eye Institute, Indianapolis, IN
  • Ryan Anderson
    Ophthalmology, Indiana University Glick Eye Institute, Indianapolis, IN
  • Yang Sun
    Ophthalmology, Indiana University Glick Eye Institute, Indianapolis, IN
  • Footnotes
    Commercial Relationships C Denwood, None; Nisha Kheradiya, None; Na Luo, None; Michael Conwell, None; Ryan Anderson, None; Yang Sun, NIH (F), American Glaucoma Society (R), Knights Templar Eye Foundation (R), Reeves Foundation (R)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3059. doi:
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    • Get Citation

      C Denwood, Nisha Kheradiya, Na Luo, Michael Conwell, Ryan Anderson, Yang Sun; PTF1a in anterior eye development in zebrafish. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3059.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

Pancreas transcription factor 1 alpha (Ptf1a) is involved in the differentiation of pancreas islet cells and may be involved in the differentation of cell types in other organ systems. In particular it has been shown to be expressed in the horizontal and amacrine cells during retinal zebrafish development. Our purpose is to determine the presence of anterior segment tissues of zebrafish during embryogenesis.

 
Methods
 

Transgenic Ptf1a:green fluorescent protein (GFP) strain zebrafish were acquired and allowed to develop for 5 days. Fish was embedded in Agarose gel and in-vivo imaging was performed. The zebrafish were scanned with 0.5 micron slices, using Zeiss confocal camera and Z-stack images were obtained.

 
Results
 

Ptf1a is transiently expressed early in the anterior segment development of zebrafish, as well as in the horizontal and amacrine cells of retina. Protein expression was evaluated and distribution of protein expression was confirmed by immunoblot analysis and immunohistochemistry.

 
Conclusions
 

In this study we show Ptf1a expression in the anterior segment during early development in the zebrafish. Future steps involve determining differentiation pathways and resulting cell types with particular attention to the inner non-pigmented ciliary epithelium and possible implications for aqueous production.

 
 
In-vivo imaging of Ptf1a expression in the anterior segment of developing transgenic Ptf1α:GFP zebrafish
 
In-vivo imaging of Ptf1a expression in the anterior segment of developing transgenic Ptf1α:GFP zebrafish
 
Keywords: 497 development • 735 trabecular meshwork • 698 retinal development  
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