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Jing Tao, Ningli Wang; Pathologic features, expression and activity of MMPs in sclera from patients with nanophthalmos. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3060.
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© ARVO (1962-2015); The Authors (2016-present)
Nanophthalmos is the leading cause of blindness in hereditary eye diseases, characterized by abnormal remodelling of scleral stroma. The purpose of this study was to investigate the mechanism of nanophthalmos by assessing the pathologic features and the role of matrix metalloproteins (MMPs) in sclera.
80 eyes of 40 patients (15 patients from 8 pedigrees and 25 sporadic patients) with nanophthalmos were studied between 1995 and 2012. 51 nanophthalmos eyes with complications during the progressive stage were treated by improved sclerectomy and sclerotomy. Sclera samples were collected from 14 patients with nanophthalmos during sclerectomy, and sclera samples from eye bank eyes were collected as normal control. Pathologic characters of the excised sclera were analyzed by immunohistochemical and electron microscopic examinations. The protein levels of MMP-1, MMP-2, MMP-3 and TIMP-1, TIMP-2 in sclera were analyzed by western-blot, and the activity level of MMP-2 was analyzed by gelatin enzymography.
Nanophthalmos is characterized with short ocular axial length (15.95±0.76mm), thick sclera (0.917±0.119mm) and ciliary body, and crowded anterior chamber, complicated with uveal effusion or/and angle-closure glaucoma during the progressive stage. The collagen fiber bundles are irregularly arranged and separated into small fibrils, and glycogen granules were found accumulated between the twisting or fraying collagen fibrils in sclera of nanophthalmos. The proteins of MMP-1, MMP-2, MMP-3 and TIMP-1, TIMP-2 were detected both in nanophthalmos and normal sclera. There was no statistically difference in the protein and activity levels between the two groups (P>0.05).
The abnormal remodelling of scleral stroma played important roles in the occurrence and development of nanophthalmos complication. No abnormal expression of MMP-1, MMP-2, MMP-3 and TIMP-1, TIMP-2 was detected in sclera from patients with nanophthalmos, according to this study.
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