June 2013
Volume 54, Issue 15
ARVO Annual Meeting Abstract  |   June 2013
Low-energy and high-frequency femtosecond laser in the production of donor corneal lamellae
Author Affiliations & Notes
  • Gustavo Victor
    Ophthalmology, University of Sao Paulo, Sao Paulo, Brazil
  • Walton Nosé
    Ophthalmology, Federal University of Sao Paulo, Sao Paulo, Brazil
  • Sidney Sousa
    Ophthalmology, University of Sao Paulo - FMRP, Ribeirao Preto, Brazil
  • Milton Alves
    Ophthalmology, University of Sao Paulo, Sao Paulo, Brazil
  • Footnotes
    Commercial Relationships Gustavo Victor, None; Walton Nosé, None; Sidney Sousa, None; Milton Alves, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3087. doi:
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      Gustavo Victor, Walton Nosé, Sidney Sousa, Milton Alves; Low-energy and high-frequency femtosecond laser in the production of donor corneal lamellae. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3087.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: This study evaluated the efficacy and reliability of a low-energy femtosecond laser with a high repetition rate in the manufacture of deep anterior and endothelial corneal lamellae

Methods: This is a prospective and laboratory investigation. Twenty-five human corneal buttons were cut tangentially to provide thick anterior lamellae (diameter, 10 mm; thickness, 500 µm). The cuts were made using an LDV® femtosecond laser in a Ziemer® anterior chamber. For a better edge, the lamellae were trephined with an 8-mm trephine (Katena®). The center thicknesses of the whole corneas and of the anterior lamellae were measured using a Mitutoyo® thickness gauge with an accuracy of 0.001 mm

Results: The thicknesses of the 25 corneas ranged from 500 to 705 µm (mean, 584 ± 51 µm). The thicknesses of the anterior lamellae ranged from 420 to 480 µm (mean, 455 ± 12.7 µm). The thicknesses of the posterior lamellae ranged from 30 to 250 µm (mean, 129 ± 52.8 µm). There were no discrepancies between the observed and expected diameters of the lamellae, and all cuts were perfectly round. The lamellar interfaces appeared regular by surgical microscopy. There were no cases of inter-lamellar adhesion

Conclusions: The LDV® femtosecond laser appears to be a safe and reliable instrument for cutting deep anterior lamellae from donor corneoscleral buttons. Even the worst-case scenario for the expected cut precision for the whole population of donated corneas would only be about ± 32 µm

Keywords: 479 cornea: clinical science • 578 laser • 741 transplantation  

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