June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Racial variation in the structure of the lamina cribrosa and sclera within 3-D fluorescent reconstructed optic nerve heads (ONH) from normal human donor tissue
Author Affiliations & Notes
  • Christopher Girkin
    Ophthalmology, Univ of Alabama at Birmingham, Birmingham, AL
  • Massimo Fazio
    Ophthalmology, Univ of Alabama at Birmingham, Birmingham, AL
  • Hongli Yang
    Devers Eye Institute, Portland, OR
  • Lan Wang
    Ophthalmology, Univ of Alabama at Birmingham, Birmingham, AL
  • Brandon Smith
    Ophthalmology, Univ of Alabama at Birmingham, Birmingham, AL
  • Chad Cheetham
    Ophthalmology, Univ of Alabama at Birmingham, Birmingham, AL
  • Claude Burgoyne
    Devers Eye Institute, Portland, OR
  • J Crawford Downs
    Ophthalmology, Univ of Alabama at Birmingham, Birmingham, AL
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3155. doi:
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      Christopher Girkin, Massimo Fazio, Hongli Yang, Lan Wang, Brandon Smith, Chad Cheetham, Claude Burgoyne, J Crawford Downs; Racial variation in the structure of the lamina cribrosa and sclera within 3-D fluorescent reconstructed optic nerve heads (ONH) from normal human donor tissue. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3155.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To examine associations between morphometric variations in the structure of the lamina cribrosa and sclera with age and race [African descent (AD), European Descent (ED)] within histologic 3-D fluorescent reconstructions of normal human donor ONHs.

 
Methods
 

The trephinated ONH and peripapillary sclera from 26 normal human donor eyes with clinical records fixed at physiologic IOP were serial sectioned using an automated microtome-based system that yielded serial fluorescent images of the block face as we cut through the ONH at 1μm section thickness (Downs JC et al, ISER 2010). We aligned and stacked the autofluorescent images to create a 3-D ONH reconstruction with a voxel resolution of 1x1x1μm and then delineated the ONH and peripapillary sclera according to guidelines set for histomorphometric ONH delineations (Downs, Yang, et al. IOVS 2007). Associations with age and race and significant interactions were determined for key morphometric parameters using mixed effects models with generalized estimating equations.

 
Results
 

In adjusted models (table), the peripapillary sclera (p=0.0032) and lamina cribrosa (p=0.00026) were thinner in eyes from AD donors, which also exhibited a deeper posterior laminar insertion (PLI) (p-value: 0.026). There was significant age-associated enlargement in Bruchs’ membrane opening (BMO) along with all neural canal landmarks within AD group (Figure).

 
Conclusions
 

Significant variations in ONH ultrastructure associated with age and across racial groups are present, which are likely to result in different biomechanical behavior with age and between racial groups.

   
Keywords: 629 optic nerve • 638 pathology: human • 577 lamina cribrosa  
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