June 2013
Volume 54, Issue 15
ARVO Annual Meeting Abstract  |   June 2013
Involvement of Angiopoietin-like 2 in Laser-induced Choroidal Neovascularization
Author Affiliations & Notes
  • Manabu Hirasawa
    Ophthalmology, Keio University, Shinjuku-ku, Japan
    Ophthalmology, Tokyo Dental College, Suidobashi Hospital, Tokyo, Japan
  • Motoyoshi Endo
    Molecular Ganesis, Kumamoto University, Kumamoto, Japan
  • Mamoru Kamoshita
    Ophthalmology, Keio University, Shinjuku-ku, Japan
  • Seiji Miyake
    Ophthalmology, Keio University, Shinjuku-ku, Japan
  • Toshio Narimatsu
    Ophthalmology, Keio University, Shinjuku-ku, Japan
  • Norihiro Nagai
    Ophthalmology, Keio University, Shinjuku-ku, Japan
  • Kazuo Tsubota
    Ophthalmology, Keio University, Shinjuku-ku, Japan
  • Yoko Ozawa
    Ophthalmology, Keio University, Shinjuku-ku, Japan
  • Footnotes
    Commercial Relationships Manabu Hirasawa, None; Motoyoshi Endo, None; Mamoru Kamoshita, None; Seiji Miyake, None; Toshio Narimatsu, None; Norihiro Nagai, None; Kazuo Tsubota, AcuFocus, Inc (C), Allergan (F), Bausch Lomb Surgical (C), Functional visual acuity meter (P), JiNS (P), Kissei (F), Kowa (F), Santen, Inc. (F), Otsuka (F), Pfizer (C), Thea (C), Echo Denki (P), Nidek (F), Ophtecs (F), Wakasa Seikatsu (F), CEPT Company (P); Yoko Ozawa, Wakasa Seikatsu.Co.,Ltd. (F), NOVARTIS Pharmacetutical Co., Ltd (F), Senju Pharmaceutical Co., Ltd. (F)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 319. doi:
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      Manabu Hirasawa, Motoyoshi Endo, Mamoru Kamoshita, Seiji Miyake, Toshio Narimatsu, Norihiro Nagai, Kazuo Tsubota, Yoko Ozawa; Involvement of Angiopoietin-like 2 in Laser-induced Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2013;54(15):319.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: Recent studies has revealed that angiopoietin-like 2 (Angptl2) has an important role in angiogenesis, chronic inflammation, and macrophage infiltration. Although these changes are involved in the pathogenesis of age-relaeted macular degeneration (AMD), little is known about Angptl2 in AMD. In this study we investigated expression of angptl2 during generation of laser-induced choroidal neovascularization (CNV) in mice.

Methods: Male C57BL/6 mice were anesthetized and CNV was induced by laser-photocoagulation. Mice were sacrificed one, two, or three days after laser injury, and eye samples were obtained. Immunohistochemistry for RPE65 (RPE specific marker) and F4/80 (macrophage specific marker) were performed with nuclear counter staining with Hoechst 33258. Real-time PCR was performed using the whole retinal pigment epithelium (RPE)-choroid complex sample to examine the mRNA levels of Angptl2, and its receptors, integrins α5, β1, and pirb, which were normalized to gapdh.

Results: In the laser-induced CNV model, Angptl2 was localized in the RPE and macrophages. Increase in the mRNA levels of integrins α5, β1, and pirb were all obvious in the whole RPE-choroid complex of this model, although the increase in Angple2 was not detected, in the early phase of CNV generation.

Conclusions: The current data proposed the possibility of Angptl2 signal’s involvement via integrin and/or pirb in the pathogenesis of CNV. Further study will unravel the role of Angptl2 in the CNV generataion.

Keywords: 453 choroid: neovascularization • 554 immunohistochemistry • 440 candidate gene analysis  

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