June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
SUMO-Conjugated RanGAP1 Is A Major Lens Protein Regulating MAP Kinase Pathway and MicroRNA Distribution
Author Affiliations & Notes
  • David Li
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
    State Key Laboratory of Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Weike Ji
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
  • Xiao-Hui Hu
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
    Key Laboratory of Protein Chemistry and Developmental Biology, College of Life Science, Hunan Normal University, Changsha, China
  • Wenfeng Hu
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
    Key Laboratory of Protein Chemistry and Developmental Biology, College of Life Science, Hunan Normal University, Changsha, China
  • Fang-Yuan Liu
    State Key Laboratory of Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Xiangcheng Tang
    State Key Laboratory of Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Kaili Wu
    State Key Laboratory of Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Yizhi Liu
    State Key Laboratory of Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Lili Gong
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
  • Mi Deng
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
  • Footnotes
    Commercial Relationships David Li, None; Weike Ji, None; Xiao-Hui Hu, None; Wenfeng Hu, None; Fang-Yuan Liu, None; Xiangcheng Tang, None; Kaili Wu, None; Yizhi Liu, None; Lili Gong, None; Mi Deng, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3192. doi:https://doi.org/
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      David Li, Weike Ji, Xiao-Hui Hu, Wenfeng Hu, Fang-Yuan Liu, Xiangcheng Tang, Kaili Wu, Yizhi Liu, Lili Gong, Mi Deng; SUMO-Conjugated RanGAP1 Is A Major Lens Protein Regulating MAP Kinase Pathway and MicroRNA Distribution. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3192. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: The nuclear Ras-related protein Ran binds guanine nucleotide (called RanGAP1) and is involved in cell cycle regulation. Whether RanGAP1 is expressed in lens and plays a role during lens differentiation remains unknown. The present study reports our initial identification of RanGAP1 as a major lens protein with important functions in regulation both signaling pathways and microRNA distribution.

Methods: Mass Spectrometry was used to identify and purify RanGAP1. Immuno-fluorescence and Western blot analysis were used to detect the expression patterns of RanGAP1 in developing mouse lens and lens epithelial cells. In vitro and in vivo experiments were used to confirm the SUMOylation regulation of RanGAP1. Lens epithelial cell differentiation and viability were analyzed in the presence or knockdown of RanGAP1. Various signal transduction pathways and microRNA distribution were analyzed in the presence or knockdown of RanGAP1.

Results: In lens systems, RanGAP1 is highly expressed in the ocular lens and lens epithelial cells. Changes in the level of RanGAP1 expression cause altered viability and differentiation pattern of lens epithelial cells. RanGAP1 is also involved in regulating MAP kinase pathway and distribution of over a dozens of microRNAs.

Conclusions: RanGAP1 is a major lens protein that executes important functions in different biological processes. Its functions is highly regulated by SUMOylation.

Keywords: 497 development • 714 signal transduction • 533 gene/expression  
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