June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
NAT2 STUDY: OMEGA-3 LEVELS IN RED BLOOD CELLS MEMBRANES CORRELATES THE PREVENTIVE EFFECT
Author Affiliations & Notes
  • Eric Souied
    Retina Creteil, University Paris Est Creteil, Creteil, France
  • Cecile Delcourt
    Retina Creteil, University Paris Est Creteil, Creteil, France
  • Giuseppe Querques
    Retina Creteil, University Paris Est Creteil, Creteil, France
  • Benedicte Merle
    Retina Creteil, University Paris Est Creteil, Creteil, France
  • Theodore Smith
    Retina Creteil, University Paris Est Creteil, Creteil, France
  • Pascale Benlian
    Retina Creteil, University Paris Est Creteil, Creteil, France
  • Footnotes
    Commercial Relationships Eric Souied, BAUSCH + LOMB (C), NOVARTIS (C), BAYER (C), THEA (C), ALLERGAN (C); Cecile Delcourt, Laboratoires Théa (F), Novartis (C), Bausch+Lomb (C); Giuseppe Querques, None; Benedicte Merle, Laboratoires Théa (F); Theodore Smith, None; Pascale Benlian, Bausch & Lomb (R)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3277. doi:
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      Eric Souied, Cecile Delcourt, Giuseppe Querques, Benedicte Merle, Theodore Smith, Pascale Benlian, NAT2 study group; NAT2 STUDY: OMEGA-3 LEVELS IN RED BLOOD CELLS MEMBRANES CORRELATES THE PREVENTIVE EFFECT. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3277.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: The NAT2 (Nutritional AMD treatment-2) study was a randomized, placebo-controlled, double-blind, parallel, comparative study. Our purpose was to evaluate the efficacy of docosahexaenoic acid (DHA) enriched oral supplementation in preventing exudative age-related macular degeneration (AMD). Here we correlated the red blood cells membrane (RBCM) levels in omega-3 and the occurrence of choroidal neovascularisation (CNV)

Methods: 263 patients with early lesions of age-related maculopathy and visual acuity better than 0.4 LogMAR units in the study eye and neovascular AMD in the fellow-eye. Patients were randomly assigned to receive either 840 mg/day DHA and 270 mg/day eicosapentaenoic acid (EPA) from fish oil capsules or the placebo (olive oil capsules) for 3 years.

Results: Time to occurrence and incidence of CNV in the study eye were not significantly different between the DHA (19.5 ± 10.9 months, 28.4%, respectively) and placebo groups (18.7 ± 10.6 months, 25.6%, respectively). EPA+DHA level significantly increased in RBCM in the DHA group (+70%; p<0.001), suggesting that DHA easily penetrated cells, but unexpectedly also in the placebo group (+9%; p=0.007). We observed a wide range of EPA+EPA levels at month 6 and year 3 in both placebo and DHA groups. In the DHA-allocated group, patients steadily achieving the highest tertile of EPA+DHA levels in RBCM had significantly lower risk (-68%; p=0.047; HR=0.32 (0.10-0.99) of developing CNV over 3 years).

Conclusions: In patients with unilateral exudative AMD, 3 years of oral DHA-enriched supplementation had the same effect on CNV incidence in the second eye as did the placebo. However, the RBCM measurements revealed that CNV incidence was significantly reduced in DHA-supplemented patients showing steadily high EPA+DHA index over 3 years.

Keywords: 412 age-related macular degeneration • 466 clinical (human) or epidemiologic studies: treatment/prevention assessment/controlled clinical trials • 424 antioxidants  
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