June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Effect of NaHS, a fast-releasing Hydrogen sulfide donor on Aqueous Humor Outflow in Porcine Trabecular Meshwork
Author Affiliations & Notes
  • Ya Fatou Njie-Mbye
    Pharmaceutical Sciences, TX Sthrn Univ-Coll of Pharm & Hlth Sci, Houston, TX
  • Jenaye Robinson
    Pharmaceutical Sciences, TX Sthrn Univ-Coll of Pharm & Hlth Sci, Houston, TX
  • Anita Okpobiri
    Pharmaceutical Sciences, TX Sthrn Univ-Coll of Pharm & Hlth Sci, Houston, TX
  • Madhura Chitnis
    Pharmaceutical Sciences, TX Sthrn Univ-Coll of Pharm & Hlth Sci, Houston, TX
  • Catherine Opere
    Department of Pharmacy Sciences, Creighton University, Omaha, NE
  • Sunny Ohia
    Pharmaceutical Sciences, TX Sthrn Univ-Coll of Pharm & Hlth Sci, Houston, TX
  • Footnotes
    Commercial Relationships Ya Fatou Njie-Mbye, None; Jenaye Robinson, None; Anita Okpobiri, None; Madhura Chitnis, None; Catherine Opere, None; Sunny Ohia, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3563. doi:https://doi.org/
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      Ya Fatou Njie-Mbye, Jenaye Robinson, Anita Okpobiri, Madhura Chitnis, Catherine Opere, Sunny Ohia; Effect of NaHS, a fast-releasing Hydrogen sulfide donor on Aqueous Humor Outflow in Porcine Trabecular Meshwork. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3563. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Evidence from our laboratory demonstrates that hydrogen sulfide (H2S) (using sodium hydrosulfide, NaHS as a generator of H2S in biological tissues) can produce pharmacological effects in mammalian ocular tissues such as a decrease in intraocular pressure (IOP) in normotensive rabbits. In the present study we tested the hypothesis that the observed IOP-lowering effect of NaHS is mediated by an increase in aqueous humor outflow in porcine trabecular meshwork.

Methods: Porcine ocular anterior segments explants containing only the trabecular meshwork were mounted on a perfusion chamber and perfused with Dulbecco’s Modified Eagle’s Medium (DMEM) at constant pressure of 7.35 mmHg and maintained at 37°C, 5% CO2. Once aqueous humor outflow was stable (~ 3 hours), ocular anterior segment explants were administered with different concentrations of NaHS (0.1 nM - 10 µM), and outflow was monitored for an additional 4 hours. Vehicle (0.1% saline) was run in parallel.

Results: The fast releasing H2S donor, NaHS (0.1 nM - 10 µM) produced a concentration-dependent increase in aqueous humor outflow in ocular anterior segment explants. For instance, administration of NaHS (100 nM) caused a significant (p<0.01) enhancement [145 ± 14.61 % of basal (mean ± SE)] of aqueous humor outflow which lasted for an hour.

Conclusions: NaHS can increase aqueous humor outflow in porcine trabecular meshwork indicating a pharmacological role for H2S in the regulation of IOP.

Keywords: 427 aqueous • 616 neurotransmitters/neurotransmitter systems • 633 outflow: trabecular meshwork  
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