June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Fluorescence multi-laser scanning microscopy of the cornea and ocular adnexa: a new era for functional confocal microscopy in ophthalmology
Author Affiliations & Notes
  • Gilles Thuret
    Ophthalmology, University Hospital of St-Etienne, Saint-Etienne, France
    Laboratory "Biology, engineering and imaging of Corneal Graft" EA2521, Faculty of Medicine, University Jean Monnet, Saint-Etienne, France
  • Marine Espinasse
    Ophthalmology, University Hospital of St-Etienne, Saint-Etienne, France
    Laboratory "Biology, engineering and imaging of Corneal Graft" EA2521, Faculty of Medicine, University Jean Monnet, Saint-Etienne, France
  • Damien Grivet
    Ophthalmology, University Hospital of St-Etienne, Saint-Etienne, France
  • Zhiguo He
    Laboratory "Biology, engineering and imaging of Corneal Graft" EA2521, Faculty of Medicine, University Jean Monnet, Saint-Etienne, France
  • Nelly Campolmi
    Ophthalmology, University Hospital of St-Etienne, Saint-Etienne, France
    Laboratory "Biology, engineering and imaging of Corneal Graft" EA2521, Faculty of Medicine, University Jean Monnet, Saint-Etienne, France
  • Jean Luc Perrot
    Dermatology, University Hospital of Saint-Etienne, Saint-Etienne, France
  • Elisa Cinotti
    Dermatology, University Hospital of Saint-Etienne, Saint-Etienne, France
  • Fabien Forest
    Laboratory "Biology, engineering and imaging of Corneal Graft" EA2521, Faculty of Medicine, University Jean Monnet, Saint-Etienne, France
    Pathology, University Hospital of Saint-Etienne, Saint-Etienne, France
  • Michel Peoc'h
    Laboratory "Biology, engineering and imaging of Corneal Graft" EA2521, Faculty of Medicine, University Jean Monnet, Saint-Etienne, France
    Pathology, University Hospital of Saint-Etienne, Saint-Etienne, France
  • Philippe Gain
    Ophthalmology, University Hospital of St-Etienne, Saint-Etienne, France
    Laboratory "Biology, engineering and imaging of Corneal Graft" EA2521, Faculty of Medicine, University Jean Monnet, Saint-Etienne, France
  • Footnotes
    Commercial Relationships Gilles Thuret, None; Marine Espinasse, None; Damien Grivet, None; Zhiguo He, None; Nelly Campolmi, None; Jean Luc Perrot, None; Elisa Cinotti, None; Fabien Forest, None; Michel Peoc'h, None; Philippe Gain, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3588. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Gilles Thuret, Marine Espinasse, Damien Grivet, Zhiguo He, Nelly Campolmi, Jean Luc Perrot, Elisa Cinotti, Fabien Forest, Michel Peoc'h, Philippe Gain; Fluorescence multi-laser scanning microscopy of the cornea and ocular adnexa: a new era for functional confocal microscopy in ophthalmology. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3588.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: In vivo confocal microscopy (IVCM) is a routine investigation for the ocular surface in reference centres. It provides high resolution pseudo histology. Nevertheless its unique laser source and imaging principle (reflectance) only provide morphological information. Multi-laser and fluorescence laser scanning microscope add a supplementary dimension by allowing the use of fluorescent markers liable to provide specific functional data

Methods: Ex vivo animal and human corneas, healthy volunteers and patients were successively examined using the multilaser vivascope 1500 (Lucid Inc, NY, MAVIG Gbmh, Germany) equipped with 3 lasers (488, 658, 785nm) and the corresponding emission (Em) filter sets. For each excitation (Ex) wavelength (λ), 3 observation modes were available: reflexion (all λ), pure reflectance (λEx = λEm), fluorescence (3 specific band pass). Ex vivo, all corneal layers were analysed without preparation and after topical application of Fluoresceine (F) and Indocyanine green (ICG) and of numerous other molecules. Topical instillation and of intraveinous injection of F and ICG were analysed in healthy volunteers and in patients

Results: Using reflexion and reflectance, the 3 Ex λ gave complementary structural informations with the highest resolution obtained at 488nm. Topical markers helped identify specific cell population and intracellular structures. Intraveinous ICG was inefficient whereas fluo provide highly contrasted conjunctival images

Conclusions: IVCM coupled with fluorescence opens a new era in the clinical imaging of the ocular surface and probably more largely in Ophthalmology. A new semeiology remains to be learned

Keywords: 596 microscopy: confocal/tunneling • 599 microscopy: light/fluorescence/immunohistochemistry • 482 cornea: epithelium  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×