Purchase this article with an account.
Cedric Blatter, Christoph Mitsch, Séverine Coquoz, Branislav Grajciar, Amardeep Singh, Rene Werkmeister, Leopold Schmetterer, Ursula Schmidt-Erfurth, Rainer Leitgeb; High resolution Doppler OCT Microangiography and angle-independent blood flow assessment in healthy and diseased retina and choroid. Invest. Ophthalmol. Vis. Sci. 2013;54(15):37.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To assess the blood flow dynamics quantitatively independent of the axial Doppler angle and to contrast the microvasculature network of the posterior segment in healthy and diseased human subjects using a bidirectional dual beam Doppler high-speed Swept Source OCT System at 1060nm
Traditional Doppler OCT is highly sensitive to motion artifacts due to the dependence on the Doppler angle, limiting its accuracy in clinical practice. To overcome this limitation, we use a bidirectional dual beam platform with flexible aligning of the incidence plane allowing reconstruction of the true flow velocity in the range of 5 to 500mm/s. The angle independent quantitative flow dynamics are extracted from specific vessel cross-sections of arteries and veins, acquired from circumpapillary and segment DOCT scan series over time at 100kA-scans/s. Microangiography is used to assess the vascular network branching and integrity. Contrast of flow towards static tissue is achieved by using an intensity difference algorithm between successive B-scans. Patches from ~12x12° acquired at selected location can resolve from small capillaries up to large vessels
The quantitative analysis profits from the intrinsic stability with respect to motion. The use of 1060nm center wavelength shows enhanced penetration into choroidal structures. The flow values in retinal vessels of healthy subjects of 20-50mm/s and 10-20mm/s for arteries and veins respectively fit well with previous findings. Highly sensitive flow contrasting has been performed at selected pathological location in human subjects suffering from choroidal neovascularisation and chronic central serous chorioretinopathy. It reveals specific alterations of the vascular network, in the choriocapillary layer in particular, like signs of vessel density loss, showing correlation with fluorescence angiography
We apply a dual beam bidirectional Doppler OCT system to accurately quantify flow dynamics and for non-invasive 3D microangiography of the posterior segment in the human eye. We present measurements performed in healthy and diseased subjects that allows deriving flow characteristic patterns of the pathological tissue. The motion artifact stable and accurate flow quantification and visualization may therefore lead ultimately to a better understanding and an enhanced early diagnosis of major retinal diseases
This PDF is available to Subscribers Only