June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Functional analysis of (pro)renin receptor in mouse retinal development
Author Affiliations & Notes
  • Atsuhiro Kanda
    Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Kousuke Noda
    Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Kenya Yuki
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Yoko Ozawa
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Takahisa Furukawa
    Molecular and Developmental Biology, Institute for Protein Research, Osaka University & JST, CREST, Suita, Japan
  • Atsuhiro Ichihara
    Department of Endocrinology and Hypertension, Tokyo Women's Medical University, Tokyo, Japan
  • Susumu Ishida
    Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Footnotes
    Commercial Relationships Atsuhiro Kanda, None; Kousuke Noda, None; Kenya Yuki, None; Yoko Ozawa, Wakasa Seikatsu.Co.,Ltd. (F), NOVARTIS Pharmacetutical Co., Ltd (F), Senju Pharmaceutical Co., Ltd. (F); Takahisa Furukawa, None; Atsuhiro Ichihara, None; Susumu Ishida, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3737. doi:https://doi.org/
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    • Get Citation

      Atsuhiro Kanda, Kousuke Noda, Kenya Yuki, Yoko Ozawa, Takahisa Furukawa, Atsuhiro Ichihara, Susumu Ishida; Functional analysis of (pro)renin receptor in mouse retinal development. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3737. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: (Pro)renin receptor [(P)RR] (also called Atp6ap2), identified as a molecule existing in the major organs but not in the circulation, has attracted growing attention due to its diverse roles in tissue renin-angiotensin system and in fundamental cellular physiology. The goal of this study is to elucidate the physiological functions of Atp6ap2/(P)RR during retinal development in mice.

Methods: Atp6ap2-floxed mice were bred with Crx-Cre transgenic mice, which expresses Cre recombinase under the control of the photoreceptor-specific cone-rod homeobox (Crx) promoter. We performed several functional and anatomical investigations including electroretinography, immunoblotting and immunohistochemistory to the conditional knockout (CKO) mice and control animals.

Results: Deletion of Atp6ap2/(P)RR did not affect retinal cell differentiation, but led to laminar disorganization in the photoreceptor layer. The visual function of rod photoreceptor cells was significantly reduced. In the CKO mice, cell adhesion and cell polarity molecules, co-localized at the apical edge of the control mouse retina, were substantially dispersed.

Conclusions: Our data suggest that Atp6ap2/(P)RR may be associated with cell adhesion and cell polarity molecules, and support its potential role in building the laminar structure during retinal development.

Keywords: 446 cell adhesions/cell junctions • 698 retinal development • 648 photoreceptors  
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