June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
The role of HGF in stabilizing blood retinal barrier of developing retina
Author Affiliations & Notes
  • JunYoung Park
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Hyun Beom Song
    Fight against Angiogenesis-Related Blindness (FARB) Laboratory, Seoul National University Hospital, Seoul, Republic of Korea
    Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Hyoung Oh Jun
    Fight against Angiogenesis-Related Blindness (FARB) Laboratory, Seoul National University Hospital, Seoul, Republic of Korea
  • Jin Hyoung Kim
    Fight against Angiogenesis-Related Blindness (FARB) Laboratory, Seoul National University Hospital, Seoul, Republic of Korea
  • Jeong Hun Kim
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Fight against Angiogenesis-Related Blindness (FARB) Laboratory, Seoul National University Hospital, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships JunYoung Park, None; Hyun Beom Song, None; Hyoung Oh Jun, None; Jin Hyoung Kim, None; Jeong Hun Kim, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3740. doi:https://doi.org/
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      JunYoung Park, Hyun Beom Song, Hyoung Oh Jun, Jin Hyoung Kim, Jeong Hun Kim; The role of HGF in stabilizing blood retinal barrier of developing retina. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3740. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: The aim of this study is to investigate the role of hepatocyte growth factor (HGF) in retinal angiogenesis and blood retinal barrier formation of developing retina.

Methods: Using the developing retina of postnatal 4, 10, 18, and 26 mice (C57BL/6), we examined the expression of HGF and its receptor, c-Met, through immunohistochemistry and immunofluorescence double staining. After treating human renal microvascular endothelial cells (HRMECs) with HGF and/or vascular endothelial growth factor (VEGF), we investigated the expression of tight junction protein (occludin and claudin-1) by Western blot analysis.

Results: The HGF and c-Met were expressed in the developing retina especially at later stage of development. It was observed that c-Met expression coincided with the vascularization of the developing retina. In addition, VEGF induced downregulation of occludin and claudin-1 was decreased by HGF in HRMECs.

Conclusions: HGF and c-Met are involved in vascularization of developing retina especially at later stage. The role of HGF in stabilizing tight junction proteins in HRMECs implicates that HGF might have a role in stabilizing blood retinal barrier at later stage of development.

Keywords: 543 growth factors/growth factor receptors • 497 development • 554 immunohistochemistry  
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