June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Retinoic acid signaling has an important role in development and maintenance of the blood retinal barrier
Author Affiliations & Notes
  • Jing Xie
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, OH
  • Alecia Cutler
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, OH
  • Mariya Ali
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, OH
  • Bela Anand-Apte
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, OH
    Department of Cell Biology, Cleveland Clinic Lerner College of Medicine at CWRU, Cleveland, OH
  • Footnotes
    Commercial Relationships Jing Xie, None; Alecia Cutler, None; Mariya Ali, None; Bela Anand-Apte, 7 183 256 B2 (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3746. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Jing Xie, Alecia Cutler, Mariya Ali, Bela Anand-Apte; Retinoic acid signaling has an important role in development and maintenance of the blood retinal barrier. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3746. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: In our previous work, we have demonstrated that the blood-retinal barrier (BRB) is established in zebrafish at 3 days postfertilization (dpf), and integrity of the BRB can be visualized in vivo using a transgenic zebrafish line, Tg(l-fabp:DBP-EGFP). The purpose of this study is to determine whether retinoic acid (RA) signaling is important for the BRB.

Methods: The l-fabp:DBP-EGFP embryos were treated with 5-20 uM DEAB or BMS 493, two specific inhibitors of the RA signaling, with or without all-trans retinoic acid (ATRA) from 2 dpf. From 5 to 7 dpf, leakage of the EGFP was detected under a fluorescent microscope. The RA signaling was also knocked down in the Tg(hsp:Cyp26a1) zebrafish to test its function the BRB.

Results: The treatment of DEAB or BMS 493, results in non-functional BRB in 63-95% fish, while 0.5 uM ATRA can effectively rescue the BRB development. Similarly, the BRB can be disrupted in the Tg(hsp:Cyp26a1) zebrafish, either at embryonic or larval stage. The expression of the claudin-5 and ZO-1, two markers of the BRB, is also decreased in the fish treated with the inhibitors.

Conclusions: These results demonstrate that the RA signaling is essential in development and maintenance of the zebrafish BRB.

Keywords: 698 retinal development • 452 choroid  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×