June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Circulating markers of retinal and optic nerve lipids
Author Affiliations & Notes
  • Niyazi Acar
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
  • Olivier Berdeaux
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
  • Zhiguo He
    EA2521, University Jean Monnet, Laboratory "Biology, Imaging, and Engineering of Corneal Grafts", Saint-Etienne, France
  • Stéphanie Cabaret
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
  • Philippe Gain
    EA2521, University Jean Monnet, Laboratory "Biology, Imaging, and Engineering of Corneal Grafts", Saint-Etienne, France
  • Gilles Thuret
    EA2521, University Jean Monnet, Laboratory "Biology, Imaging, and Engineering of Corneal Grafts", Saint-Etienne, France
  • Catherine Garcher
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
    University Hospital, Department of Ophthalmology, Dijon, France
  • Alain Bron
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
    University Hospital, Department of Ophthalmology, Dijon, France
  • Lionel Bretillon
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3757. doi:
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    • Get Citation

      Niyazi Acar, Olivier Berdeaux, Zhiguo He, Stéphanie Cabaret, Philippe Gain, Gilles Thuret, Catherine Garcher, Alain Bron, Lionel Bretillon; Circulating markers of retinal and optic nerve lipids. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3757.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

Blood lipids are frequently used as a surrogate of lipid composition of peripheral tissues. Even if it is well accepted, such a relationship has never been clearly demonstrated for the eye. The aim of this study was to determine in human samples whether a lipidomic approach based on red blood cells could reveal associations between circulating and ocular lipid profiles.

 
Methods
 

Red blood cells, retinas and optic nerves were collected from 9 human donors. The lipidomic analyses on these tissues were done by both, gas chromatography and liquid chromatography coupled to an electrospray ionization source-mass spectrometer (LC-ESI-MS/MS).

 
Results
 

Gas chromatographic approach did not show any relevant association between circulating and ocular lipids except for arachidonic acid whose circulating amounts were positively associated with its retinal and optic nerve levels. However, significant associations emerged from LC-ESI-MS/MS analyses. Indeed, phospholipid species in red blood cells were positively or negatively associated with representative pools of retinal DHA (docosahexaenoic acid), retinal VLC-PUFA (very-long chain polyunsaturated fatty acids) or optic nerve plasmalogens.

 
Conclusions
 

First, our results show that LC-ESI-MS/MS methodology is more appropriate than gas chromatography for lipidomics on red blood cells, and further extrapolation to ocular lipids. Second, this study has identified several individual lipid species that can be good candidates to represent circulating biomarkers of ocular lipids.

 
Keywords: 583 lipids • 467 clinical laboratory testing • 695 retinal degenerations: cell biology  
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