June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Role of Periostin in Choroidal Fibrovascular Membrane Formation
Author Affiliations & Notes
  • Takahito Nakama
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Shigeo Yoshida
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Keijiro Ishikawa
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Ryo Asato
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Takeshi Kita
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Shintaro Nakao
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Yukio Sassa
    Ophthalmology, Kyushu University, Fukuoka, Japan
    Ophthalmology, Fukuoka University Chikushi Hospital, Fukuoka, Japan
  • Yuji Oshima
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Hiroshi Enaida
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Tatsuro Ishibashi
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Footnotes
    Commercial Relationships Takahito Nakama, None; Shigeo Yoshida, None; Keijiro Ishikawa, None; Ryo Asato, None; Takeshi Kita, None; Shintaro Nakao, None; Yukio Sassa, None; Yuji Oshima, None; Hiroshi Enaida, None; Tatsuro Ishibashi, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3855. doi:
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    • Get Citation

      Takahito Nakama, Shigeo Yoshida, Keijiro Ishikawa, Ryo Asato, Takeshi Kita, Shintaro Nakao, Yukio Sassa, Yuji Oshima, Hiroshi Enaida, Tatsuro Ishibashi; Role of Periostin in Choroidal Fibrovascular Membrane Formation. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3855.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: The pathogenesis of choroidal fibrovascular membrane (FVM) formation remains unclear. Periostin is a matricellular protein involved in tissue and vascular remodeling. We previously reported the increased expression of periostin in FVMs obtained from patients with proliferative diabetic retinopathy (Yoshida et al IOVS 2011). The purpose of this study was to investigate the role of periostin in choroidal FVM formation.

Methods: We generated mouse laser-induced choroidal neovascularization model (CNV) using C57BL/6 wild type (WT) and periostin knock out (KO) mice. The concentration of periostin in retinal pigment epithelium (RPE)/choroid complex from WT CNV mice were measured by ELISA. Periostin localization in choroidal FVM obtained from CNV mice and from patients with age-related macular degeneration (AMD) was evaluated by immunohistochemical analysis. We also quantified the volume of CNV and the degree of fibrosis in FVM using choroidal flat mount of WT and periostin KO mice at 7 or 21 days after laser injury.

Results: ELISA showed that periostin concentrations in mouse CNV increased and reached a peak at 14 days after laser coagulation. Immunohistochemical analysis showed that periostin was expressed in RPE cells in choroidal FVM of both CNV mice and AMD patients. At 7 days after laser injury, the volume of CNV and the degree of fibrosis didn’t differ between WT and periostin KO mice. At 21 days after laser injury, the amount and density of collagen type 1 in FVM were significantly reduced in periostin KO mice compared to WT mice (p<0.01), while the volume of CNV was not significantly altered.

Conclusions: These results suggest that periostin plays a role in fibrosis of choroidal FVM formation.

Keywords: 453 choroid: neovascularization • 519 extracellular matrix • 654 proliferation  
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