Purchase this article with an account.
Nicolas Bonnin, Loic Blanchon, Corinne Belville, Georges Souteyrand, Frederic Chiambaretta, Vincent Sapin; Retinoids improvements during the treatment by human amniotic membrane of corneal alkali burns in a mouse model. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3870.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
The aim of this study is to establish the effects of a human amniotic membrane (HAM) treatment by retinoids (active derivatives of vitamin A); just before its graft on a corneal alkali burn using a mouse model.
This protocol was approved by the regional ethic committee (CEMEA Auvergne). Thirty eyes of male mice, dispatched into 5 groups: the first group was untreated, the others received a treatment by HAM grafts prepared according to four different conditions and one control: non cultivated (control group), cultivated during 24 hours in a standard culture medium, cultivated in a culture medium containing all-trans retinoic acid (ATRA) dissolved in dimethylsulfoxide (DMSO) and cultivated in a culture medium containing DMSO. Induction of HAM by ATRA was assessed by PCR quantification of RARβ (already published as a gene induced by retinoids). The photographs evaluated corneal re-epithelialization, histopathology evaluated ulceration depth, immunohistochemical analysis and signal quantification numbered the expression of MMP9 and VEGF.
For the ulcers surface, we noted a significant difference between the groups HAM cultivated during 24 hours without or with ATRA compared to the untreated group; and any significant difference between the group HAM cultivated in a medium containing DMSO and the untreated group. Concerning the ulcers depth, there was a significant difference between the group treated by HAM cultivated 24 hours with ATRA compared to the untreated group. Immunohistochemistry assays showed that MMP 9 and VEGF expression were decreased in the groups treated with HAM cultivated during 24 hours with ATRA compared to the other groups.
The positive effects of HAM culture show its biological effect more than its mechanical effect. DMSO appears to reduce the effect of ATRA, implying quickly the use of a new non-toxic vector for clinical applications. Nevertheless, our results clearly showed that the induction of MAH by ATRA appears to have a beneficial effect on the ulcers depth and the expression of MMP 9 and VEGF. Our preliminary results are really promising in order to propose a positive engineering of HAM applied for wound healing graft on cornea in the next future.
This PDF is available to Subscribers Only