June 2013
Volume 54, Issue 15
ARVO Annual Meeting Abstract  |   June 2013
Proteome study of keratocytes after PRGF-Endoret treatment
Author Affiliations & Notes
  • Francisco Jose Muruzabal
    Biotechnology Institute, Vitoria, Spain
  • Gorka Orive
    Biotechnology Institute, Vitoria, Spain
  • Eduardo Anitua
    Biotechnology Institute, Vitoria, Spain
  • Footnotes
    Commercial Relationships Francisco Jose Muruzabal, Biotechnology Institute (E); Gorka Orive, BTI Biotechnology Institute (E); Eduardo Anitua, Biotechnology Institute (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3888. doi:
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      Francisco Jose Muruzabal, Gorka Orive, Eduardo Anitua; Proteome study of keratocytes after PRGF-Endoret treatment. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3888.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: Plasma rich in growth factors (PRGF®-Endoret®) is an autologous technology that contains a pool of proteins specifically addressed for wound healing and tissue regeneration. Treatment with PRGF-Endoret eye drops stimulates those cells close to damage area to proliferate, migrate and differentiate with the aim of promoting the regeneration of injured cornea. The aim of this study was to characterize the proteins and signaling pathways involved in the keratocyte activation after PRGF-Endoret treatment.

Methods: Blood from healthy donors was collected, centrifuged and Plasma Rich in Growth Factors (PRGF-Endoret) was prepared avoiding the buffy coat. Primary human keratocytes were incubated with PRGF-Endoret eye drops at different times of treatment, collected and analyzed using a proteomic approach that combines 2-DE followed by MALDI-TOF/TOF.

Results: The deregulated proteins obtained at different time points were grouped into families and networks according to gene ontology. The proteomic analysis of human keratocytes treated with PRGF-Endoret revealed a wide range of deregulated proteins. These proteins are mainly associated with signaling pathways related to the regulation of cell death, proliferation, cytoskeletal and motor proteins, and wound healing, among others.

Conclusions: A wide range of proteins and signaling pathways were deregulated after PRGF-Endoret treatment. We found an enrichment of both proteins and protein families specifically involved in tissue regeneration and wound healing.

Keywords: 484 cornea: stroma and keratocytes • 658 protein purification and characterization • 765 wound healing  

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