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Noelia Andollo, Vanesa Freire, Arturo Grau, Jaime Etxebarria, Juan Duran, Maria-Celia Morales; Effect of human albumin in combination of blood derivatives rich in growth factors in the wound healing capability of corneal epithelial cells. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3892. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Blood derivatives rich in growth factors are good promoters of corneal wound healing. Our aim is to test in vitro the capability of human albumin as a synergic compound combined with a blood derivative rich in growth factors to improve the healing rate of corneal epithelial cells.
In vitro proliferation and wound healing experiments were performed using the human corneal epithelial HCE cell line. We studied the following treatments: 1) 20% Serum of Plasma Rich in Growth Factors (s-PRGF) 2) 50% s-PRGF 3) 10% Human serum albumin (albumin) 4) 20% s-PRGF+10% albumin 5) 50% s-PRGF+10% albumin 6) 10% FBS and 7) 1% bovine serum albumin (BSA) as control. To manufacture s-PRGF whole blood was collected by venipuncture from healthy volunteers.
The results show that human albumin induces highly significant higher proliferation of HCE cells than any dose of s-PRGF after 72 hours of treatment. Moreover, no significant differences are found among human albumin, 20% s-PRGF+albumin and 50% s-PRGF+albumin. Conversely, 20% and 50% s-PRGF treatments improve cell migration with respect to the rest of the treatments, with statistically significant differences. Treatments of albumin, 20% s-PRGF+albumin and 50% s-PRGF+albumin do not show significant differences among them.
Human albumin by itself induces higher proliferation of epithelial corneal cells but lower cell migration, compared with the effect of the blood derivative s-PRGF. In addition, combination of both does not improve proliferation and affects negatively migration.
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