June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Effect of human albumin in combination of blood derivatives rich in growth factors in the wound healing capability of corneal epithelial cells
Author Affiliations & Notes
  • Noelia Andollo
    Cell Biology And Histology, University of The Basque Country, BioCruces Health Research Institute, Leioa, Spain
  • Vanesa Freire
    Cell Biology And Histology, University of The Basque Country, BioCruces Health Research Institute, Leioa, Spain
    R & D Dept., Instituto Clínico-Quirúrgico de Oftalmología, Bilbao, Spain
  • Arturo Grau
    Hospital Sótero del Rio, Santiago, Chile
  • Jaime Etxebarria
    Cell Biology And Histology, University of The Basque Country, BioCruces Health Research Institute, Leioa, Spain
    University Hospital of Cruces, BioCruces Health Research Institute, Barakaldo, Spain
  • Juan Duran
    Ophthalmology, University of the Basque Country, BioCruces Health Research Institute, Leioa, Spain
    R & D Dept., Instituto Clínico-Quirúrgico de Oftalmología, Bilbao, Spain
  • Maria-Celia Morales
    University Hospital of Cruces, BioCruces Health Research Institute, Barakaldo, Spain
  • Footnotes
    Commercial Relationships Noelia Andollo, None; Vanesa Freire, None; Arturo Grau, None; Jaime Etxebarria, bioftalmik (C); Juan Duran, None; Maria-Celia Morales, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3892. doi:https://doi.org/
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      Noelia Andollo, Vanesa Freire, Arturo Grau, Jaime Etxebarria, Juan Duran, Maria-Celia Morales; Effect of human albumin in combination of blood derivatives rich in growth factors in the wound healing capability of corneal epithelial cells. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3892. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Blood derivatives rich in growth factors are good promoters of corneal wound healing. Our aim is to test in vitro the capability of human albumin as a synergic compound combined with a blood derivative rich in growth factors to improve the healing rate of corneal epithelial cells.

Methods: In vitro proliferation and wound healing experiments were performed using the human corneal epithelial HCE cell line. We studied the following treatments: 1) 20% Serum of Plasma Rich in Growth Factors (s-PRGF) 2) 50% s-PRGF 3) 10% Human serum albumin (albumin) 4) 20% s-PRGF+10% albumin 5) 50% s-PRGF+10% albumin 6) 10% FBS and 7) 1% bovine serum albumin (BSA) as control. To manufacture s-PRGF whole blood was collected by venipuncture from healthy volunteers.

Results: The results show that human albumin induces highly significant higher proliferation of HCE cells than any dose of s-PRGF after 72 hours of treatment. Moreover, no significant differences are found among human albumin, 20% s-PRGF+albumin and 50% s-PRGF+albumin. Conversely, 20% and 50% s-PRGF treatments improve cell migration with respect to the rest of the treatments, with statistically significant differences. Treatments of albumin, 20% s-PRGF+albumin and 50% s-PRGF+albumin do not show significant differences among them.

Conclusions: Human albumin by itself induces higher proliferation of epithelial corneal cells but lower cell migration, compared with the effect of the blood derivative s-PRGF. In addition, combination of both does not improve proliferation and affects negatively migration.

Keywords: 765 wound healing • 482 cornea: epithelium  
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