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Benjamin Frankfort, Meike van der Heijden, Cameron Cowan, Samuel Wu; Acute Intraocular Pressure Elevation in Mice Results in Diminished Scotopic and Photopic Contrast Sensitivity. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3927.
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To assess the relationship between acute elevations of intraocular pressure (IOP) and visual contrast sensitivity in mice.
Baseline scotopic and photopic contrast sensitivities were determined on peak spatiotemporal frequency (spatial frequency = 0.08 cyc/deg; temporal frequency = 2 deg/sec; speed = 25 cyc/sec) for five (5) c57Bl6 female mice with an established optokinetic-based technique. IOP was then elevated experimentally in one eye via a single anterior chamber injection of highly cohesive sodium hyaluronate (Healon 5, Abbott Medical Optics, Inc.). After post-operative measurement of IOP with a rebound tonometer (Tonolab) mice were immediately dark-adapted. Following two hours of dark-adaptation contrast sensitivity was assessed with the same technique under scotopic and then photopic conditions. IOP and contrast sensitivity were sequentially measured on post-operative days 1, 2, 3, 4, and 6. T-tests and repeated measures ANOVAs with cutoffs of p < 0.05 were conducted in SPSS version 21 (IBM).
IOP is elevated to approximately 35 mmHg following a single injection of sodium hyaluronate but decreases to baseline after about 2 days. When IOP is elevated, both scotopic and photopic contrast sensitivities are markedly reduced. Both scotopic and photopic contrast sensitivities recover as IOP normalizes. There is a mild delay in both scotopic and photopic contrast sensitivity recovery which is more prominent under photopic conditions.
Acute IOP elevation results in decreased scotopic and photopic contrast sensitivity in mice. IOP normalizes faster than contrast sensitivity recovers.
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