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Dong Hyun KIM, Sung Wook Park, Byung Joo Lee, Dong Hyun Jo, Jin Hyoung Kim, Jeong Hun Kim; The role of endothelial progenitor cell in the tumor angiogenesis of retinoblastoma. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3971.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the role of cancer stem cell and endothelial progenitor cell in angiogenesis of orthotopic transplantation mouse model of retinoblastoma.
Regarding orthotopic transplantation mouse model of retinoblastoma, SNUOT-Rb1 cells (1x107) were intravitreally-inoculated into the eyes of the BALB/c nude mice. Four weeks after inoculation, the mice were sacrificed and the eyes were enucleated. To identify tumor development, H&E staining was performed in 4 um section slides. Immunofluorescence staining was performed on mouse retinoblastoma tissue to evaluate the spatial distribution of endothelial progenitor cell and tumor vessel using CD133 and CD34 as endothelial progenitor cell markers and collagen type IV as endothelial cell marker.
The Wintersteiner rosettes were identified in mouse retinoblastoma tissue with SNUHOT-Rb1 cells. CD34 and CD133 were co-expressed in the Wintersteiner rosettes. CD133 was expressed in the cells with collagen type IV consisting tumor vessels.
Our data demonstrated that orthotopic transplantation mouse model of retinoblastoma was established by intravitreous injection of SNUHOT-Rb1 cells. Endothelial progenitor cells might contribute tumor angiogenesis in retinoblastoma.
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