June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
The role of endothelial progenitor cell in the tumor angiogenesis of retinoblastoma
Author Affiliations & Notes
  • Dong Hyun KIM
    Fight against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
    Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Sung Wook Park
    Fight against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
    Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Byung Joo Lee
    Fight against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
    Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Dong Hyun Jo
    Fight against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
    Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Jin Hyoung Kim
    Fight against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • Jeong Hun Kim
    Fight against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
    Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships Dong Hyun KIM, None; Sung Wook Park, None; Byung Joo Lee, None; Dong Hyun Jo, None; Jin Hyoung Kim, None; Jeong Hun Kim, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3971. doi:
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    • Get Citation

      Dong Hyun KIM, Sung Wook Park, Byung Joo Lee, Dong Hyun Jo, Jin Hyoung Kim, Jeong Hun Kim; The role of endothelial progenitor cell in the tumor angiogenesis of retinoblastoma. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3971.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To investigate the role of cancer stem cell and endothelial progenitor cell in angiogenesis of orthotopic transplantation mouse model of retinoblastoma.

 
Methods
 

Regarding orthotopic transplantation mouse model of retinoblastoma, SNUOT-Rb1 cells (1x107) were intravitreally-inoculated into the eyes of the BALB/c nude mice. Four weeks after inoculation, the mice were sacrificed and the eyes were enucleated. To identify tumor development, H&E staining was performed in 4 um section slides. Immunofluorescence staining was performed on mouse retinoblastoma tissue to evaluate the spatial distribution of endothelial progenitor cell and tumor vessel using CD133 and CD34 as endothelial progenitor cell markers and collagen type IV as endothelial cell marker.

 
Results
 

The Wintersteiner rosettes were identified in mouse retinoblastoma tissue with SNUHOT-Rb1 cells. CD34 and CD133 were co-expressed in the Wintersteiner rosettes. CD133 was expressed in the cells with collagen type IV consisting tumor vessels.

 
Conclusions
 

Our data demonstrated that orthotopic transplantation mouse model of retinoblastoma was established by intravitreous injection of SNUHOT-Rb1 cells. Endothelial progenitor cells might contribute tumor angiogenesis in retinoblastoma.

  
Keywords: 703 retinoblastoma • 748 vascular endothelial growth factor • 744 tumors  
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