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Bin Li; The role of Osteopontin expression in the mechanism of invasion and metastasis potential in Uveal Melanoma. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4222.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the relationship between Osteopontin(OPN) expression with invasion and metastasis potential in UM.
(1)50 UM samples were collected in Beijing Tongren Hospital. The OPN expression of the samples was detected by using immunohistochemistry staining. (2)The expression of OPN mRNA in different metastasis potential uveal melanoma cell lines (MUM-2B,C918,OCM-1A) was analyzed by using Q-PCR. (3)To bulid up OPN siRNA lentiviral vector and transfect it into UM cell lines C918 and MUM-2B to detect the proliferation and cell invasion capacity of cells by using MTT and Transwell method.
(1)OPN was positively expressed in 10/13 patients with metastasis and 14/37 patients without metastasis (P=0.000). There were 11 samples and 13 samples exhibiting the positive expression in 15 epithelial cell type and 35 non-epithelial cell type of UM respectively (P=0.000). The OPN positive expression were 20/30 affected tumors and 4/20 unaffected ciliary tumors(P=0.000). No significant differences in OPN expression were found between age, gender, eyes, largest basal diameters and (P=0.536, 0.256, 0.802, 0.848, 0.555).(2)The relative expression value of mRNA of OPN was in turn 1±0.04, 0.91±0.03, 0.08±0.01 in MUM-2B,C918,OCM-1A (P<0.05). The OPN mRNA expression was significant enhanced in high metastatic potential cell lines(MUM-2B,C918) compared with low metastatic potential cell lines OCM-1A.(P<0.05), but no significant difference was observed between two high metastatic potential cell lines (P=0.804). (3)OPN siRNA lentiviral vector transfected UM cell lines MUM-2B,C918, the transfection efficiency is over 80%, and the expression level of the OPN is lower than that before transfection ( P<0.05).(4)After transfection of OPN siRNA lentiviral vector, the cell proliferation capacity of C918 cells in Konck Down group is lower than cells in Negative Control group and Control group, while the cell proliferation capacity of MUM-2B cells in Konck Down group is a little lower than cells in the other two groups. (5) After transfection of OPN siRNA lentiviral vector, the cell invasion capacity of C918 cells in Konck Down group is lower than cells in other two groups.
OPN expression is associated with invasion and metastasis potential in uveal melanoma cell line. The results imply that OPN expression maybe as an indicator of uveal melanoma invasion and metastasis ability.
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