June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
In vivo contrast-enhanced high-frequency ultrasonography of experimental uveal melanoma: imaging features and histopathologic correlations
Author Affiliations & Notes
  • Hans Grossniklaus
    Dept of Ophthal, School of Med, Emory University, Atlanta, GA
  • Shin Kang
    Dept of Ophthal, School of Med, Emory University, Atlanta, GA
  • Qing Zhang
    Dept of Ophthal, School of Med, Emory University, Atlanta, GA
  • Footnotes
    Commercial Relationships Hans Grossniklaus, None; Shin Kang, None; Qing Zhang, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 4229. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Hans Grossniklaus, Shin Kang, Qing Zhang; In vivo contrast-enhanced high-frequency ultrasonography of experimental uveal melanoma: imaging features and histopathologic correlations. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4229. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

To evaluate the utility of in vivo imaging of a rabbit model of uveal melanoma utilizing high-frequency contrast-enhanced ultrasound (HF-CE-US) with 2- or 3- dimensional modes, and to correlate the sonographic findings with histopathologic characteristics.

 
Methods
 

Five New Zealand white rabbits were inoculated into their right eyes with aliquots of 92.1 human uveal melanoma cells. The rabbits were immune suppressed with subcutaneous cyclosporine 15mg/kg qd from three days prior to inoculation to four weeks after inoculation. Tumor-bearing eyes were imaged using HF-CE-US to determine the 2- and 3-dimensional tumor size and relative blood volume. The rabbits were sacrificed and their eyes with melanoma were histologically examined to determine melanoma size and mean vascular density (MVD).

 
Results
 

Melanoma grew in the choroid in all eyes. Utilizing HF-CE-US, melanomas were visualized as relatively hyperechoic regions in the images. The correlation coefficient of sonographic size or volume compared with histologic area were r square =0.72 and 0.70, respectively. The sonographic tumor relative blood volume correlated with histologic tumor vascularity (MVD) (r=0.92).

 
Conclusions
 

There is a positive correlation between in vivo sonographic tumor volume/size and histologic tumor size in our rabbit ocular melanoma model. HF-CE-US corresponds to microvascular density and blood volume. HF-CE-US is a real-time, non-invasive method for evaluation of intraocular melanoma tumor area and relative blood volume.

 
 
Rabbit model of uveal melanoma demonstrating fundus view of melanoma, histology of melanoma, ultrasound and HF-CE-US
 
Rabbit model of uveal melanoma demonstrating fundus view of melanoma, histology of melanoma, ultrasound and HF-CE-US
 
 
Correlation between HF-CE-US tumor area and histologic tumor size (r squared=0.70)
 
Correlation between HF-CE-US tumor area and histologic tumor size (r squared=0.70)
 
Keywords: 589 melanoma • 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 637 pathology: experimental  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×