June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Monophasic PDSAg-induced EAU dominates over relapsing R14-induced EAU
Author Affiliations & Notes
  • Ulrike Kaufmann
    Ophthalmology, Clinic of the Ludwig-Maximilians-University, Munich, Germany
  • Maria Diedrichs-Möhring
    Ophthalmology, Clinic of the Ludwig-Maximilians-University, Munich, Germany
  • Gerhild Wildner
    Ophthalmology, Clinic of the Ludwig-Maximilians-University, Munich, Germany
  • Footnotes
    Commercial Relationships Ulrike Kaufmann, None; Maria Diedrichs-Möhring, None; Gerhild Wildner, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 4521. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Ulrike Kaufmann, Maria Diedrichs-Möhring, Gerhild Wildner; Monophasic PDSAg-induced EAU dominates over relapsing R14-induced EAU. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4521. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Rat EAU can be induced with S-Ag peptide PDSAg, inducing monophasic, or IRBP peptide R14, inducing relapsing uveitis. We have previously shown different dynamics of intraocular T cell populations during the two types of EAU, but the exact mechanisms behind the disease courses remain elusive. Here we used different combinations of the two antigens for immunization to investigate the mutual influence on the disease course and the immune response.

Methods: EAU was induced with PDSAg or R14 in CFA and with combinations of both, either administered separately at contralateral sides or as a mixture of both. Disease course was analyzed daily and cytokine pattern (IFN-γ, IL-17, IL-10) and Foxp3 expression of intraocular cells was determined at onset, peak, resolution and late remission of disease by flow cytometry. Data of the combined immunizations were compared with that from the conventional PDSAg and R14 immunizations.

Results: While in R14-induced EAU 75% of the eyes developed relapses, none of the PDSAg- or PDSAg/R14-mixture immunized rats had recurrences. However, contralateral administration of both antigens allowed relapses in 12.5% of eyes. The cytokine pattern of intraocular cells looked similar in those animals immunized with both antigens, irrespective of the application, but differed from the pattern of the rats which were immunized with PDSAg or R14 only. Rats immunized with both antigens showed an R14-like cytokine pattern at onset of EAU and a PDSAg-like cytokine expression at resolution of EAU. Cultivated lymph node cells of mixture-immunized rats had increased numbers of Foxp3+, but decreased IFN-γ+ cells compared to rats immunized with PDSAg or R14 only, respectively.

Conclusions: Disease course and cytokine pattern of intraocular cells confirmed a dominant role of the monophasic, PDSAg-specific immune response. Increased regulatory T cells expressing IL-10 or Foxp3 might be responsible for the prevention of relapses.

Keywords: 746 uveitis-clinical/animal model • 490 cytokines/chemokines • 555 immunomodulation/immunoregulation  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×