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Ghulam Mohammad, Ahmed Abu El-Asrar; Matrix metalloproteinase-9 regulates angiogenic and vasculogenic factors in diabetic retina. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4600. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the role of matrix metalloproteinase-9 (MMP9) in the regulation of angiogenic and vasculogenic factors in the retinas of experimental diabetic mice.
A group of wild-type (WT) and MMP9 knockout mice (MMP9-KO) were made diabetic by intraperitional injection of Streptozotacin for five consective days. Three months after induction of diabetes, mice were sacrificed, the retinas were removed and analyzed for the expressions of MMP9, vascular endothelial growth factor (VEGF), soluble vascular endothelial growth factor receptor-2 (VEGFR-2), stromal cell derived factors-1 (SDF-1), the chemokine SDF-1 receptor CXCR4, stem cell factor (SCF), and prominin-1 (CD133) by RT-PCR , Western blot analysis and immunohistochemistry
Diabetes of ~3 months in WT mice significantly increased the expression of MMP9, VEGF, sVEGFR -2, SDF-1, CXCR4, and SCF and decreased CD133 expression in the retinas compared to WT nondiabetic. Retinas of MMP9-KO mice were protected from diabetes-induced upregulation of MMP9, VEGF, VEGFR -2, SDF-1, CXCR4, and SCF and downregulation of CD133 compared to WT diabetic mice.
MMP9 may play an important role in retinal neovascularization by regulating the expression of angiogenic and vasculogenic factors in diabetic retina
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