June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Dephosphorylation of CREB Regulates Its Functions on Lens Differentiation
Author Affiliations & Notes
  • Xiao-Hui Hu
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
    Ket Laboratory of Protein Chemistry & Developmental Biology, College of Life Science, Hunan Normal University, Changsha, China
  • Wenfeng Hu
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
    Ket Laboratory of Protein Chemistry & Developmental Biology, College of Life Science, Hunan Normal University, Changsha, China
  • Weike Ji
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
  • Mi Deng
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
  • Lili Gong
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
  • Zachary Woodward
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
  • Wenbin Liu
    Ket Laboratory of Protein Chemistry & Developmental Biology, College of Life Science, Hunan Normal University, Changsha, China
  • Shaojun Liu
    Ket Laboratory of Protein Chemistry & Developmental Biology, College of Life Science, Hunan Normal University, Changsha, China
  • David Li
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE
    Ket Laboratory of Protein Chemistry & Developmental Biology, College of Life Science, Hunan Normal University, Changsha, China
  • Footnotes
    Commercial Relationships Xiao-Hui Hu, None; Wenfeng Hu, None; Weike Ji, None; Mi Deng, None; Lili Gong, None; Zachary Woodward, None; Wenbin Liu, None; Shaojun Liu, None; David Li, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 462. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Xiao-Hui Hu, Wenfeng Hu, Weike Ji, Mi Deng, Lili Gong, Zachary Woodward, Wenbin Liu, Shaojun Liu, David Li; Dephosphorylation of CREB Regulates Its Functions on Lens Differentiation. Invest. Ophthalmol. Vis. Sci. 2013;54(15):462. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Our previous studies have shown that serine/threonine phosphatases-1 and -2A are major lens phosphatases. Inhibition or knockdown of PP-1 and PP-2A activities led to microphthalmia in goldfish and zebrafish. However, the exact mechanisms by which PP-1 and PP-2A regulate lens differentiation remains to be investigated. In the present study, we present evidence to show that PP-1 and PP-2A directly dephosphorylate CREB to regulate lens differentiation.

Methods: Human embryonic lens epithelial cells (FHL-124), mouse lens epithelial cells, wild type and CREB knockout mice were used as testing systems. Co-immunoprecipitation assays were used to investigate the interactions between CREB and PP-1/-2A. Treatment with various kinase inhibitors, and overexpression and knockdown of PP-1 or -2A were used to examine their effects on CREB phosphorylation status. QRT-PCR, Western-blot analysis and reporter gene activity assays were used to study the roles of CREB under various conditions of phosphorylation/dephosphorylation.

Results: Various kinases including PKA, PKC and ERK are implicated in phosphorylating CREB in the ocular lens. Both PP-1 and PP-2A are found capable of dephosphorylating CREB to modulate its transcription activity.

Conclusions: PP-1 and -2A dephosphorylate CREB to modulate its function in lens differentiation. (Supported by EY018380, CSC and HNU)

Keywords: 739 transcription factors • 646 phosphorylation • 497 development  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×