Purchase this article with an account.
Min Gu Kang, So Young Lee, Na Rae Kim, Hee Seung Chin; Effect of HMG-CoA reductase inhibitor(statins) on the expression of catalase in human retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4620.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To evaluate the effects of HMG-CoA reductase inhibitor(statins) on the expression of catalase in human retinal pigment epithelial cells.
RPE cells were incubated for 6 hours with various concentrations (0, 1, 2.5, 5, 10µM) of simvastatin. After 6 hours of incubation, real time PCR was peformed to evaluate the expression of catalase mRNA and western blot was performed to evaluate the expression of catalase in RPE cells.
Catalase and catalase mRNA were maximally expressed when the human RPE cells treated with 10µM of simvastatin for 6 hours. Also the human RPE cells showed concentration-dependent expression of catalase and catalase mRNA when treated with concentrations of simvastatin for 6 hours.
Simvastatin upregulates the expression of catalase in human RPE cells. Simvastatin may have some clinical benefits in preventing retinal diseases associated with oxidative stress, such as age-related macular degeneration.
This PDF is available to Subscribers Only