June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Inhibition of retinal neovascularization by luteolin via suppression of VEGF expression and VEGFR signaling pathway
Author Affiliations & Notes
  • Sung Wook Park
    FARB, Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
    Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Chang Sik Cho
    FARB, Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Hyoung Oh Jun
    FARB, Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Nam Hee Ryu
    Diabetic Complications Research Center, Division of Traditional Korean Medicine Integrated Research, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea
  • Jin Hyoung Kim
    FARB, Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Young Yu
    FARB, Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Jin Sook Kim
    Diabetic Complications Research Center, Division of Traditional Korean Medicine Integrated Research, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea
  • Jeong Hun Kim
    FARB, Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
    Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships Sung Wook Park, None; Chang Sik Cho, None; Hyoung Oh Jun, None; Nam Hee Ryu, None; Jin Hyoung Kim, None; Young Yu, None; Jin Sook Kim, None; Jeong Hun Kim, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 4652. doi:https://doi.org/
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      Sung Wook Park, Chang Sik Cho, Hyoung Oh Jun, Nam Hee Ryu, Jin Hyoung Kim, Young Yu, Jin Sook Kim, Jeong Hun Kim; Inhibition of retinal neovascularization by luteolin via suppression of VEGF expression and VEGFR signaling pathway. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4652. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: This study was to investigate the anti-angiogenic effect of luteolin against reactive oxygen species (ROS) induced retinal neovascularization

Methods: The toxicity of luteolin was evaluated through modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in human retinal microvascular endothelial cells (HRMECs) as well as terminal deoxynucleotidyl transferase dUTP nick-end labeling staining in the retina of C57BL/6J. After intravitreal injection of luteolin in the mouse model of ROP, retinal neovascularization was examined by fluorescence angiography and vessel counting. Anti-angiogenic activity of luteolin was evaluated by VEGF-induced migration and tube formation assay. The effect of luteolin on t-BH-induced ROS production was measured with 2’7’-dichlorofluorescein diacetate. The effect of luteolin on t-BH induced and hypoxia-induced VEGF transcription and expression were evaluated by RT-PCR and Western blot, respectively.

Results: Luteolin never affected the viability of HRMECs up to 10 μM, where luteolin never induce any structural change in all retinal layers. Luteolin inhibited retinal neovascularization in the mouse model of ROP. Moreover, VEGF-induced migration and tube formation were significantly decreased by co-treatment of luteolin. Luteolin attenuated VEGF transcription via blockade of t-BH induced ROS production. Luteolin suppressed hypoxia-induced VEGF expression via attenuating hypoxia inducible factor 1 α expression.

Conclusions: Our results suggest that luteolin could be a potent anti-angiogenic agent for retinal neovascularization, which is related to anti-oxidative activity to block ROS production and to subsequently suppress VEGF expression and the pro-angiogenic effect of VEGF

Keywords: 700 retinal neovascularization • 548 hypoxia • 634 oxidation/oxidative or free radical damage  
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