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Yoshinori Oie, Satoru Andojo, Natsumi Konno, Hiroshi Takayanagi, Takayuki Nozaki, Shizu Takeda, Takeshi Soma, Motokazu Tsujikawa, Kohji Nishida; Air transport system of oral mucosal tissue and cell sheets for clinical trial. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4679. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To develop air transport system of oral mucosal tissue and cell sheets for clinical trial using autologous tissue-engineered oral mucosal epithelial cell sheet.
We developed a transport container that can maintain and monitor the inside temperature and air pressure. Rabbit oral mucosal tissue was transported from Osaka University to Tohoku University with this container. Epithelial cell sheet was fabricated with the transported tissue, and was transported from Tohoku University to Osaka University. The tissue and cell sheets were evaluated before and 12 hours after transportation. Histological and immunohistochemical analyses were performed. Cell viability and cell purity were evaluated by flow cytometry.
Temperature inside the container was kept 7 to 10 degrees Celsius for tissue, and 32 to 36 degrees Celsius for cell sheets. Air pressure was kept 990 to 1015 hPa during transportation. Basal cells in the tissue were positive for p63. And cell sheets were well stratified and harvested successfully after transport. The expression patterns of keratin 3/76, p63, and ZO-1were equivalent before and after transport. The cell viability was 72.6% before transport and 73.1% after transport. Epithelial purity was 82.0% before transport and 89.9% after transport.
OralOra mucosal tissue and epithelial cell sheets can be transported using this new transport system with quality maintenance.
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