June 2013
Volume 54, Issue 15
ARVO Annual Meeting Abstract  |   June 2013
A synthetic carrier for cultured corneal endothelium implantation
Author Affiliations & Notes
  • Mark Daniell
    CERA, Melbourne, VIC, Australia
  • Karl Brown
    CERA, Melbourne, VIC, Australia
  • Berkay Ozcelik
    Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, VIC, Australia
  • Penelope McKelvie
    Anatomical Pathology, St. Vincent's Hospital, Melborne, VIC, Australia
  • Hong Zhang
    CERA, Melbourne, VIC, Australia
  • Keren Abberton
    The O'Brien Institute, Fitzroy, VIC, Australia
  • Footnotes
    Commercial Relationships Mark Daniell, None; Karl Brown, None; Berkay Ozcelik, None; Penelope McKelvie, None; Hong Zhang, None; Keren Abberton, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 4687. doi:
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      Mark Daniell, Karl Brown, Berkay Ozcelik, Penelope McKelvie, Hong Zhang, Keren Abberton, CERA Surgical Research Unit; A synthetic carrier for cultured corneal endothelium implantation. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4687.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: Purpose A tissue engineered corneal endothelium could overcome potential problems associated with donor tissues. We have developed a thin (50µm) synthetic hydrogel film suitable for corneal endothelial cell (CEC) culture and implantation. An ovine model was used to develop and evaluate the film in vitro and in vivo.

Methods: Methods Synthetic hydrogel films (SHF) with superior properties were developed using a novel approach. Ovine CEC (OCEC) density in vivo was determined by specular microscopy and compared to cell density achieved on tissue culture plastic and the hydrogel film as determined using Computer Assisted Steriological Toolbox (Leica). Films were implanted into an ovine anterior chamber for 30d before histological evaluation.

Results: OCEC density in vivo was determined to be 3150cells/mm2 in 14 month old merino sheep (n=3). Cell density at 28d was 3150 (SEM 459) on SHF, 2530 (SEM 526) on collagen I coated SHF and 3770 (SEM 201) tissue culture plastic (n=4). The films had sufficient tensile strength for implantation and readily un-scrolled within the anterior chamber. Histology determined that PHF did not produce and inflammatory response in vivo (n=4).

Conclusions: Hydrogel films suitable for implantation that support CEC cultivation have been produced. A combination of a SFH with a cultured CEC monolayer may be able to replace the donor tissue lenticule used in Descemet’s Stripping Automated Endothelial Keratoplasty.

Keywords: 481 cornea: endothelium  

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