June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Benzalkonium chloride accelerates amyloid fibril formation in corneal dystrophies in vitro
Author Affiliations & Notes
  • Yuichi Kaji
    Ophthalmology, University of Tsukuba, Ibaraki, Japan
    Laboratory of Protein Folding, Institute for Protein Research Osaka University, Osaka, Japan
  • Hisashi Yagi
    Laboratory of Protein Folding, Institute for Protein Research Osaka University, Osaka, Japan
  • Yusuke Kato
    Laboratory of Protein Folding, Institute for Protein Research Osaka University, Osaka, Japan
  • Yuji Goto
    Laboratory of Protein Folding, Institute for Protein Research Osaka University, Osaka, Japan
  • Tetsuro Oshika
    Ophthalmology, University of Tsukuba, Ibaraki, Japan
  • Footnotes
    Commercial Relationships Yuichi Kaji, None; Hisashi Yagi, None; Yusuke Kato, None; Yuji Goto, None; Tetsuro Oshika, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 4736. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Yuichi Kaji, Hisashi Yagi, Yusuke Kato, Yuji Goto, Tetsuro Oshika; Benzalkonium chloride accelerates amyloid fibril formation in corneal dystrophies in vitro. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4736. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Corneal dystrophies are genetic disorders resulting in progressive corneal clouding due to amyloid fibril formation derived from the transforming growth factor β-induced (TGFBI) gene. Amyloid fibril formation is influenced by the presence of solvents and surfactants such as sodium dodecyl sulfate (SDS). In the present study, we aimed to reveal the role of benzalkonium chloride (BAC) and SDS in amyloid fibril formation of TGFBI-derived peptides in vitro.

Methods: Various concentrations of BAC or SDS were added to solutions of synthetic peptides corresponding to wild-type, Avellino corneal dystrophy, and lattice corneal dystrophy. The time course of the amount of formed amyloid fibrils in the solution was quantitatively measured using thioflavin T. In addition, the seeding effect of amyloid fibril formation was evaluated in the presence of BAC and SDS.

Results: For all synthetic peptides, BAC and SDS accelerated amyloid fibril formation in both de novo and seeding models at 0.01 to 0.5 mM and 0.1 to 1.5 mM, respectively. BAC accelerated amyloid fibril formation in the in vitro models of corneal dystrophies.

Conclusions: The result indicates that most of the eye drops containing BAC may deteriorate corneal dystrophies. Eye drops that do not contain BAC would be preferred for patients with corneal dystrophies

Keywords: 484 cornea: stroma and keratocytes • 480 cornea: basic science • 659 protein structure/function  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×