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Juan Reynaud, Brad Fortune; A method for mapping retinal nerve fiber layer birefringence. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4844.
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© ARVO (1962-2015); The Authors (2016-present)
We have shown that RNFL retardance, measured by scanning laser polarimetry (SLP), declines prior to RNFL thickness measured by spectral domain optical coherence tomography (SDOCT) using global average peripapillary scan locations for non-human primates (NHP) with experimental glaucoma (EG). These results are consistent with the hypothesis that axonal cytoskeletal disruption precedes axon bundle thinning within the RNFL during the course of glaucomatous neurodegeneration. An important step forward in testing this hypothesis is to develop a method for mapping RNFL birefringence beyond the conventional peripapillary scan locations.
SLP scans (GDxVCC, Carl Zeiss Meditec, Inc) and SDOCT cube scans consisting of 290 horizontal raster lines (Spectralis, Heidelberg Engineering, GmbH) were acquired longitudinally in NHPs with EG. Custom software was developed to rapidly co-localize pairs of reflectance images and derive birefringence maps.
Fig1 demonstrates each step of the method and an example of glaucomatous change in RNFL birefringence.
We developed software to rapidly and effectively co-localize the reflectance image from SLP scans to the reflectance image from SDOCT scans and to derive RNFL birefringence maps from the retardance and thickness values, respectively. Registration of scans over time and across instruments reduces noise in longitudinal series of sectoral data.
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