June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Resveratrol Protects Human Retinal Pigment Epithelial Cells from Inflammatory Insults
Author Affiliations & Notes
  • R Kutty
    National Eye Institute, National Institutes of Health, Bethesda, MD
  • Chandra Nagineni
    National Eye Institute, National Institutes of Health, Bethesda, MD
  • William Samuel
    National Eye Institute, National Institutes of Health, Bethesda, MD
  • Todd Duncan
    National Eye Institute, National Institutes of Health, Bethesda, MD
  • Camasamudram Vijayasarathy
    National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD
  • Cynthia Jaworski
    National Eye Institute, National Institutes of Health, Bethesda, MD
  • T. Michael Redmond
    National Eye Institute, National Institutes of Health, Bethesda, MD
  • Footnotes
    Commercial Relationships R Kutty, None; Chandra Nagineni, None; William Samuel, None; Todd Duncan, None; Camasamudram Vijayasarathy, None; Cynthia Jaworski, None; T. Michael Redmond, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 4989. doi:https://doi.org/
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      R Kutty, Chandra Nagineni, William Samuel, Todd Duncan, Camasamudram Vijayasarathy, Cynthia Jaworski, T. Michael Redmond; Resveratrol Protects Human Retinal Pigment Epithelial Cells from Inflammatory Insults. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4989. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Inflammatory responses of retinal pigment epithelium (RPE) are implicated in the pathogenesis of age-related macular degeneration (AMD). RPE cells in culture respond to the proinflammatory cytokines IFN-γ, TNF-α and IL-1β by increasing the expression of chemokines and cytokines. Resveratrol, a naturally occurring polyphenol, is a well characterized anti-inflammatory antioxidant. The purpose of the present study is to investigate whether resveratrol can modulate the effect of IFN-γ, TNF-α and IL-1β on RPE cells.

Methods: Confluent cultures of ARPE-19 cells were treated with a proinflammatory cytokine mixture containing IFN-γ, TNF-α and IL-1β in a serum free medium for various time intervals. Total RNA fraction was then isolated and real-time PCR analysis of gene expression was performed using TaqMan reagents (Applied Biosystems) with GAPDH as an endogenous control. The activation of nuclear factor-κB (NFκB) was examined by western immunoblot analysis of cell extracts using anti-phospho-NFκB p65 (Ser536) antibody.

Results: Pretreatment of ARPE-19 cells with resveratrol (50 µM) effectively blocked the induction of CCL5, CXCL9, CSF2 and NOS2A (inducible form of nitric oxide synthase) by the proinflammatory cytokines (IFN-γ + TNF-α + IL-1β). Resveratrol also prevented the decrease in the expression of HMOX1 (heme oxygenase-1) in cells exposed to proinflammatory cytokines. Immunoblot analysis showed that the phospo-NFκB p65 increased in cells in response to the treatment with proinflammatory cytokines. This increase in phosphorylation was considerably reduced in the presence of resveratrol.

Conclusions: Resveratrol attenuated the inflammatory response of the RPE cells in culture by its ability to block the activation of NFκB signaling pathway by the proinflammatory cytokines. Thus, resveratrol, due to its ability to protect RPE from inflammatory insults, may have therapeutic potential as a nutraceutical in arresting the development of ocular degenerative diseases such as AMD.

Keywords: 557 inflammation • 701 retinal pigment epithelium • 424 antioxidants  
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