June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
In vitro Uptake and Release of Natamycin Dex-b-PLA Nanoparticles from Silicone Hydrogel Contact Lens Materials
Author Affiliations & Notes
  • Chau-Minh Phan
    Centre for Contact Lens Research, School of Optometry and Vision Science, University of Waterloo, Waterloo, ON, Canada
  • Lakshman Subbaraman
    Centre for Contact Lens Research, School of Optometry and Vision Science, University of Waterloo, Waterloo, ON, Canada
  • Lyndon Jones
    Centre for Contact Lens Research, School of Optometry and Vision Science, University of Waterloo, Waterloo, ON, Canada
  • Shengyan Liu
    Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada
  • Frank Gu
    Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada
  • Footnotes
    Commercial Relationships Chau-Minh Phan, None; Lakshman Subbaraman, None; Lyndon Jones, Alcon (F), Alcon (R), Allergan (F), Abbott Medical Optics (R), Bausch & Lomb (R), Ciba Vision (F), Ciba Vision (R), CooperVision (F), Johnson & Johnson (F), Johnson & Johnson (R); Shengyan Liu, None; Frank Gu, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 501. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Chau-Minh Phan, Lakshman Subbaraman, Lyndon Jones, Shengyan Liu, Frank Gu; In vitro Uptake and Release of Natamycin Dex-b-PLA Nanoparticles from Silicone Hydrogel Contact Lens Materials. Invest. Ophthalmol. Vis. Sci. 2013;54(15):501.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To evaluate the uptake and release of the antifungal agent natamycin encapsulated within poly(D,L-lactide)-dextran nanoparticles (Dex-b-PLA NPs) from model silicone hydrogel contact lens materials.

Methods: Six model contact lens materials (gel A: poly(hydroxyethyl methacrylate, pHEMA); gel B: 85% pHEMA: 15% [Tris(trimethylsiloxy)silyl]-propyl methacrylate (TRIS); gel C: 75% pHEMA: 25% TRIS; gel D: 85% N,N dimethylacrylamide (DMAA): 15% TRIS; gel E: 75% DMAA: 25% TRIS; gel F: DMAA) were prepared using photoinitiation. The resulting lens materials were incubated in two conditions: (1) natamycin dissolved in deionized (DI) water, and (2) natamycin encapsulated within Dex-b-PLA NPs in 9.1 % dimethylsulfoxide (DMSO)/DI water for 7 days (d) at 25±3 oC. The release of natamycin from these materials in 2 mL of unpreserved saline solution, pH 7.4 at 32±2 oC was monitored using UV-Visible spectrophotometry at 304 nm over 7 d. The release solution was replenished every 24 hours (h).

Results: The uptake of natamycin by all model lens materials increased between 1 and 7 d (p<0.001). There were no differences in drug uptake between lens materials containing pHEMA and DMAA. However, the uptake of natamycin encapsulated with NPs was higher than the uptake of the drug dissolved in DI water (p<0.05). The release of the drug was observed to be higher in materials containing DMAA than pHEMA (p<0.05). Additionally, all gels loaded with natamycin NPs also released more drug compared to gels soaked with natamycin in DI water (p<0.001). The drug release by all materials reached equilibrium within 12 h. After replenishing the release solution every 24 h, gels loaded with natamycin NPs continued to release drugs for up to 4 d (p<0.05).

Conclusions: Model contact lens materials loaded with drug-Dex-b-PLA NPs provides a drug delivery vehicle capable of releasing natamycin for up to 12 h. In addition, materials containing DMAA-TRIS may be more suitable for drug delivery of natamycin due to the higher drug release observed with these materials.

Keywords: 477 contact lens • 422 antibiotics/antifungals/antiparasitics • 607 nanotechnology  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×