Purchase this article with an account.
Hsi-Kung Kuo, Yi-Hao Chen, Pei-Chang Wu; Pharmacodynamic study of Intravitreal liposomal doxorubicin by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI/MS). Invest. Ophthalmol. Vis. Sci. 2013;54(15):5055.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
to investigate the pharmacodynamics of doxorubicin and liposomal doxorubicin (Lipo-Dox) after intravitreal injection.
Pigmented rabbits were used. One eye accepted intravitreal injection of doxorubicin or Lipo-Dox (10 μg/ml). Another eye was injected with 0.1 ml BSS as the control. To evaluate the pharmacodynamic change of the drugs, the animals were sacrificed on the day 1, 3, 5, 7 and 14 after the injection. The vitreous contents and retina extracts were prepared for LC/MS/MS and MALDI/MS study. On the matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI/IMS) group, the full eye sections are prepared from cryo-section.
Pharmacodynamic study by MALDI/MS showed liposomal doxorubicin existed in the vitreous for at least 14 days with the highest density on day 7. The result is compatible with the slowly released property of liposomal drugs. To study the drug distribution with MALDI/IMS was failed due to background interference.
Intravitreal injection of liposomal doxorubicin can obtain a sustained drug concentration for at least 14 days with the peak on day7.
This PDF is available to Subscribers Only