June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Intravitreal injection of acyclovir causes dose-related retinal toxicity in rabbits
Author Affiliations & Notes
  • Gabriela Ioshimoto
    Department Experimental Psychology, Psychology Institute, University of Sao Paulo, São Paulo, Brazil
  • Beatriz Sayuri Takahashi
    Department of Ophthalmology, University of Sao Paulo, Sāo Paulo, Brazil
  • Cristiano Pessoa
    Department of Cell and Developmental Biology, Biomedical Sciences Institute, University of Sao Paulo, São Paulo, Brazil
  • Andre Liber
    Department Experimental Psychology, Psychology Institute, University of Sao Paulo, São Paulo, Brazil
  • Balázs Nagy
    Department Experimental Psychology, Psychology Institute, University of Sao Paulo, São Paulo, Brazil
  • Dora Ventura
    Department Experimental Psychology, Psychology Institute, University of Sao Paulo, São Paulo, Brazil
  • Francisco Max Damico
    Department of Ophthalmology, University of Sao Paulo, Sāo Paulo, Brazil
  • Footnotes
    Commercial Relationships Gabriela Ioshimoto, None; Beatriz Sayuri Takahashi, None; Cristiano Pessoa, None; Andre Liber, None; Balázs Nagy, None; Dora Ventura, None; Francisco Max Damico, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5090. doi:https://doi.org/
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      Gabriela Ioshimoto, Beatriz Sayuri Takahashi, Cristiano Pessoa, Andre Liber, Balázs Nagy, Dora Ventura, Francisco Max Damico; Intravitreal injection of acyclovir causes dose-related retinal toxicity in rabbits. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5090. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Acute retinal necrosis is a rapidly progressive and devastating viral retinitis caused by the herpes virus family. Although systemic acyclovir is the treatment of choice, intravitreal injection of acyclovir may be used an adjuvant therapy during the first 2 days of treatment, while systemically administered acyclovir has not reached therapeutic levels in the retina. The aim of this study is to determine the functional effects of acyclovir in the rabbit retina after intravitreal injection.

Methods: Acyclovir (0.1, 1, and 10 mg) was injected in the right eye of 30 New Zealand albino rabbits and sterile saline solution was injected in the left eye as control. Electroretinograms (ERGs) were recorded 1 day before and 2, 7, and 14 days after the intravitreal injection ERGs were recorded according to a modified ISCEV protocol. Data were analyzed comparing the four different pre- and post-treatment time points and treated and the non-treated eyes (Wilcoxon rank test).

Results: No statistically significant differences were found in the eyes injected with 0.1 mg. Eyes injected with 1 mg showed significant reduction in a- and b-wave amplitudes (day 2 and days 2 and 7, respectively) (p<0.05). Differences were not present in day 15. Eyes injected with 10 mg presented significant reduction in a- and b-wave from days 2 to 15 (p<0.05). No differences were found in implicit time.

Conclusions: ERG results suggest that intravitreal injection of acyclovir in rabbits may cause dose-related toxicity to photoreceptors and post-receptoral cells. Functional changes caused by acyclovir 1 mg are reversible by day 15, but 10 mg causes irreversible changes. These findings suggest that intravitreal injection of acyclovir is a potential treatment for viral retinitis.

Keywords: 510 electroretinography: non-clinical • 503 drug toxicity/drug effects • 688 retina  
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