June 2013
Volume 54, Issue 15
ARVO Annual Meeting Abstract  |   June 2013
Proteomic Analysis of the Keratitis Associated Pseudomonas aeruginosa
Author Affiliations & Notes
  • Abby Sewell
    Ophthalmology Research, Summa Health System, Akron, OH
  • Jeffrey Dunmire
    Ophthalmology Research, Summa Health System, Akron, OH
  • Michael Wehmann
    Ophthalmology Research, Summa Health System, Akron, OH
  • Rachida Bouhenni
    Ophthalmology Research, Summa Health System, Akron, OH
  • Footnotes
    Commercial Relationships Abby Sewell, None; Jeffrey Dunmire, None; Michael Wehmann, None; Rachida Bouhenni, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5213. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Abby Sewell, Jeffrey Dunmire, Michael Wehmann, Rachida Bouhenni; Proteomic Analysis of the Keratitis Associated Pseudomonas aeruginosa. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5213.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: To compare the proteomic profile of a clinical isolate of Pseudomonas aeruginosa (P. aeruginosa) obtained from an infected cornea of a contact lens wearer (corneal strain) and a non-corneal strain, ATCC 10145.

Methods: Phenotypic assays of P. aeruginosa such as twitching motility, biofilm formation and antibiotic sensitivity tests were performed using standard methods. Whole protein lysates were analyzed by Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) in triplicate and relative protein abundances were determined by spectral counting. G test followed by post hoc Holm Sidak was used for statistical analyses to determine significance in the differential expression of proteins between the two strains.

Results: LC-MS/MS revealed significant differences in protein composition between the two strains. A total of 585 proteins were detected. Among these 73 were up or down regulated in the corneal strain compared to ATCC10145 and 44 were detected only in the corneal strain. Proteins that were detected explicitly in the corneal strain were involved in different functional groups including cell motility and secretion, lipid metabolism, cell envelope biogenesis and ion transport and metabolism.In addition to a number of hypothetical proteins which had similarities to proteins involved in iron storage, type IV secretion associated lipoproteins, proteases and secreted cytotoxins. Proteins that were significantly upregulated in the corneal strain compared to the non-corneal strain included those involved in motility (flagellin type B, 21fold, p=0.0003), polyhydroxyalkanoate synthesis (PhaF, 11fold, p=0.02), chorismate biosynthesis (chorismate synthase, 8fold, p=0.007) and virulence (lipotoxin F, 3fold, p=0.004). The non ribosomal peptide synthetases (NRPS) were the fourth most abundant proteins in the corneal strain and were missing in ATCC 10145.

Conclusions: Results from this study confirm that the keratitis associated P. aeruginosa strain is pathogenic and expresses a unique protein profile indicative of phenotypic adaptations to its environment. Identification of the protein profile of the corneal strain of P. aeruginosa in this study will aid in the elucidation of novel intervention strategies to reduce the burden of P. aeruginosa infection in keratitis.

Keywords: 573 keratitis • 664 pseudomonas • 663 proteomics  

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.