June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Proteomic analysis of the interaction Fusarium solani - Staphylococcus epidermidis isolated from human corneal ulcers
Author Affiliations & Notes
  • Mariana Ortiz-Casas
    Microbiology and Proteomics, Institute of Opthalmology "Conde de Valenciana", Mexico City, Mexico
  • Aida Rodríguez-Tovar
    Medical Mycology, Escuela Nacional de Ciencias Biológicas, IPN, Mexico City, Mexico
  • Juan Pablo Reyes-Grajeda
    Medical Proteomics Unit, Instituto Nacional de Medicina Genómica, Mexico City, Mexico
  • Herlinda Mejia-Lopez
    Microbiology and Proteomics, Institute of Opthalmology "Conde de Valenciana", Mexico City, Mexico
  • María Martínez-Rivera
    Medical Mycology, Escuela Nacional de Ciencias Biológicas, IPN, Mexico City, Mexico
  • Luis Bautista-Hernández
    Microbiology and Proteomics, Institute of Opthalmology "Conde de Valenciana", Mexico City, Mexico
  • Carolina Gaona-Juárez
    Microbiology and Proteomics, Institute of Opthalmology "Conde de Valenciana", Mexico City, Mexico
  • Nadia Luz López-Espinosa
    Microbiology and Proteomics, Institute of Opthalmology "Conde de Valenciana", Mexico City, Mexico
  • Victor Bautista
    Microbiology and Proteomics, Institute of Opthalmology "Conde de Valenciana", Mexico City, Mexico
  • Footnotes
    Commercial Relationships Mariana Ortiz-Casas, None; Aida Rodríguez-Tovar, None; Juan Pablo Reyes-Grajeda, None; Herlinda Mejia-Lopez, None; María Martínez-Rivera, None; Luis Bautista-Hernández, None; Carolina Gaona-Juárez, None; Nadia Luz López-Espinosa, None; Victor Bautista, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5233. doi:https://doi.org/
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      Mariana Ortiz-Casas, Aida Rodríguez-Tovar, Juan Pablo Reyes-Grajeda, Herlinda Mejia-Lopez, María Martínez-Rivera, Luis Bautista-Hernández, Carolina Gaona-Juárez, Nadia Luz López-Espinosa, Victor Bautista; Proteomic analysis of the interaction Fusarium solani - Staphylococcus epidermidis isolated from human corneal ulcers. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5233. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To study the proteomic profile of co-culture of Fusarium solani and Staphylococcus epidermidis in comparison with mono-cultures.

Methods: Fusarium solani and Staphylococcus epidermidis were isolated from human corneal ulcers. The microorganisms were characterized by microbiological and molecular technics. Mono-cultures and co-cultures were established in Müller-Hinton Agar. Plates containing 72-h biomass were processed to obtain the cellular extract; proteins were quantified and purified before two-dymensional electrophoresis (2D) was performed. The proteomic profiles were analyzed by the software Dymension 2 to determine the differential expression of the co-culture compared with the mono-cultures. The spots that showed differential expression were identified by mass spectrometry using MALDI-TOF 4800 (ABsciex).

Results: The fungi growth was lower in the co-cultures than in monocultures (p <0.0001). Proteomic profiles from the mono-cultures and co-cultures showed 40 proteins with differential expression; 21 of these were identified and classified as: metabolism (13), defense mechanisms (4) and pathogenic/antagonism traits (4). The majority of the identified proteins corresponding to metabolic proteins and also the majority of them were down-regulated. These down-regulated proteins suggest that the metabolism of both microorganisms is in “stand-by”, while they are adapting to share the same space, because the defense mechanisms proteins are up-regulated. As it is known, the bacteria grew faster and it propitiated the acid conditions that the fungi needed to grow. While this was happening, the fungi had a low metabolism that it is reflected in the proteins involved in energy metabolic pathways.

Conclusions: The proteomic profile of the co-culture Fusarium solani-Staphylococcus epidermidis showed differential expression compared with the mono-cultures proteomic profiles. The majority of the proteins with differential expression were identified as enzymes that participate in important metabolic pathways, suggesting that the co-culture is a cooperative interaction instead of competitive. Two proteins, FBA and enolase, could be possible biomarkers.

Keywords: 480 cornea: basic science • 530 fungal disease • 663 proteomics  
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