June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Hydrogen-Loaded Eye Drops Suppress Progression of Keratoconus-Like Corneal Lesion of SKC Mouse
Author Affiliations & Notes
  • Naoko Kato
    Ophthalmology, National Defense Medical College, Saitama, Japan
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Yuichi Uchino
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Emi Inagaki
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Shigeo Ohta
    Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Graduate School of Medicine, Nippon Medical School, Kanagawa, Japan
  • Kazuo Tsubota
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5292. doi:
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      Naoko Kato, Yuichi Uchino, Emi Inagaki, Shigeo Ohta, Kazuo Tsubota; Hydrogen-Loaded Eye Drops Suppress Progression of Keratoconus-Like Corneal Lesion of SKC Mouse. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5292.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Keratoconus is a congenital and idiopathic corneal disease that occurs in puberty and progresses until third or fourth decades. The exact etiology of keratoconus is not clarified; however, involvement of oxidative stress in the pathogenesis of keratoconus has recently been reported. SKC mouse is an inbred line of spontaneous mutant mice with corneas resembling human keratoconus. More than 90 % of male skc/stm mice develop protrusion of cornea until 3 months of age. Molecular hydrogen is a novel antioxidant to efficiently reduce oxidative stress. Instillation of hydrogen-loaded eye drops is reported to attenuate retinal ischemia-reperfusion damage or corneal angiogenesis after alkali-burn. We evaluated in the present investigation whether instillation of hydrogen-loaded physiological saline eye drops attenuates the progression of keratoconus-like corneal lesion of SKC mouse.

Methods: Male SKC mice were divided into 2 groups. Seven mice (group H) were instilled hydrogen-loaded physiological saline eye drops on the right eye. Hydrogen-loaded eye drops were prepared by dissolving H(2) gas into a saline to saturated level and administered to the ocular surface 5 times per day from 3 weeks to 12 weeks of age. Other 7 mice (group P) were instilled normal physiological saline eye drops. We took photographs of their cornea every week, and compared the frequency of occurrance of keratoconus-like corneal lesion up to 24 weeks of age.

Results: The frequency of keratoconus-like lesion was 28.6% at 6th week, 57.1% at 7th week, 71.4% at 9th week, 85.7% at 16th week, and stable thereafter in the group P, and 14.3% at 6th week, 28.6% at 10th week, 42.9% at 12th week, 57.1% at 16th week, and not increased thereafter in the group H. The frequency of keratoconus-like lesion was significantly higher in group P from 6th to 9th week of age (p<0.05).

Conclusions: Hydrogen-loaded phosphate saline eye drops impeded and suppressed progression of keratoconus-like corneal lesion in SKC mouse.

Keywords: 424 antioxidants • 484 cornea: stroma and keratocytes • 574 keratoconus  
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