June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
ROCK Inhibitor Y-27632 Enhances the Mucin Secretion from Goblet Cells Derived from Limbal Stem Cells
Author Affiliations & Notes
  • Won Seok Choi
    Ophthalmology, Hallym University College of Medicine, SEOUL, Republic of Korea
  • Eui Sang Chung
    Ophthalmology, Sungkyunkwan University School of Medicine, SEOUL, Republic of Korea
  • Tae-Young Chung
    Ophthalmology, Sungkyunkwan University School of Medicine, SEOUL, Republic of Korea
  • Joon Young Hyon
    Ophthalmology, Seoul National University College of Medicine, SEOUL, Republic of Korea
    Ophthalmology, Seoul National University Bundang Hospital, SEOUL, Republic of Korea
  • Won Ryang Wee
    Ophthalmology, Seoul National University College of Medicine, SEOUL, Republic of Korea
  • Young Joo Shin
    Ophthalmology, Hallym University College of Medicine, SEOUL, Republic of Korea
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5410. doi:
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      Won Seok Choi, Eui Sang Chung, Tae-Young Chung, Joon Young Hyon, Won Ryang Wee, Young Joo Shin; ROCK Inhibitor Y-27632 Enhances the Mucin Secretion from Goblet Cells Derived from Limbal Stem Cells. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5410.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To investigate the effect of Rho-associated coiled kinase inhibitor (ROCK inhibitor) Y-27632 on Goblet cells derived from corneal limbal stem cells.

Methods: Goblet cells were derived from human limbal stem cells and cultured. The cells were treated with ROCK inhibitor. Periodic acid-Schiff (PAS) staining and immunofluorescence staining of ABCG2, CK-7 and MUC5AC was performed. Cell proliferation rate was measured with BrdU labeling assay.

Results: The cultured cells were stained with ABCG2 and CK-7. Goblet cell derived from limbal stem cells showed mucin secretion by PAS staining and MUC5AC staining. Cell proliferation rate increased with ROCK inhibitor.

Conclusions: Goblet cells were differentiated from limbal stem cells. ROCK inhibitor enhanced mucin secretion and proliferation rate in Goblet cells derived from limbal stem cells.

Keywords: 480 cornea: basic science  
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