June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
ROCK Inhibitor Y-27632 Enhances the Mucin Secretion from Goblet Cells Derived from Limbal Stem Cells
Won Seok Choi; Eui Sang Chung; Tae-Young Chung; Joon Young Hyon; Won Ryang Wee; Young Joo Shin
Author Affiliations & Notes
  • Won Seok Choi
    Ophthalmology, Hallym University College of Medicine, SEOUL, Republic of Korea
  • Eui Sang Chung
    Ophthalmology, Sungkyunkwan University School of Medicine, SEOUL, Republic of Korea
  • Tae-Young Chung
    Ophthalmology, Sungkyunkwan University School of Medicine, SEOUL, Republic of Korea
  • Joon Young Hyon
    Ophthalmology, Seoul National University College of Medicine, SEOUL, Republic of Korea
    Ophthalmology, Seoul National University Bundang Hospital, SEOUL, Republic of Korea
  • Won Ryang Wee
    Ophthalmology, Seoul National University College of Medicine, SEOUL, Republic of Korea
  • Young Joo Shin
    Ophthalmology, Hallym University College of Medicine, SEOUL, Republic of Korea
  • Footnotes
    Commercial Relationships Won Seok Choi, None; Eui Sang Chung, None; Tae-Young Chung, None; Joon Young Hyon, None; Won Ryang Wee, None; Young Joo Shin, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5410. doi:
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      Won Seok Choi, Eui Sang Chung, Tae-Young Chung, Joon Young Hyon, Won Ryang Wee, Young Joo Shin; ROCK Inhibitor Y-27632 Enhances the Mucin Secretion from Goblet Cells Derived from Limbal Stem Cells. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5410.

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Abstract

Purpose: To investigate the effect of Rho-associated coiled kinase inhibitor (ROCK inhibitor) Y-27632 on Goblet cells derived from corneal limbal stem cells.

Methods: Goblet cells were derived from human limbal stem cells and cultured. The cells were treated with ROCK inhibitor. Periodic acid-Schiff (PAS) staining and immunofluorescence staining of ABCG2, CK-7 and MUC5AC was performed. Cell proliferation rate was measured with BrdU labeling assay.

Results: The cultured cells were stained with ABCG2 and CK-7. Goblet cell derived from limbal stem cells showed mucin secretion by PAS staining and MUC5AC staining. Cell proliferation rate increased with ROCK inhibitor.

Conclusions: Goblet cells were differentiated from limbal stem cells. ROCK inhibitor enhanced mucin secretion and proliferation rate in Goblet cells derived from limbal stem cells.

Keywords: 480 cornea: basic science  
© 2013, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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