June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Tear Film Proteins Strongly-Adherent to a Non-Ionic Silicone Hydrogel Contact Lens Across the Wear Cycle
Author Affiliations & Notes
  • Michael Foster
    Vision Care, Alcon, Duluth, GA
  • Alan Landers
    Vision Care, Alcon, Duluth, GA
  • Manal Gabriel
    Vision Care, Alcon, Duluth, GA
  • Jennifer Lane
    Vision Care, Alcon, Duluth, GA
  • Walter Nash
    Vision Care, Alcon, Duluth, GA
  • Footnotes
    Commercial Relationships Michael Foster, Alcon, a Novartis Company (E); Alan Landers, Alcon (E); Manal Gabriel, Alcon, A Novartis company (E); Jennifer Lane, Alcon Laboratories (E), Novartis (E); Walter Nash, ALCON, a Novartis Company (E)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5489. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Michael Foster, Alan Landers, Manal Gabriel, Jennifer Lane, Walter Nash; Tear Film Proteins Strongly-Adherent to a Non-Ionic Silicone Hydrogel Contact Lens Across the Wear Cycle. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5489.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To evaluate the difference between day one versus day twenty-eight levels of eight strongly-adherent contact lens-adsorbed protein analytes (lysozyme, lactoferrin, lipocalin, free κ/λ Ig light chain, IgA heavy chain, monomeric IgA, secretory component, and total protein) among a group of non-ionic silicone hydrogel contact lens wearers.

Methods: Lenses were collected from subjects aseptically and stored at below -20 degrees C. All subjects wore the same non-ionic silicone hydrogel lens material and used the same lens care regimen. Lenses were equilibrated to ambient temperature and rinsed in 2 mL of phosphate buffered saline for 5 minutes with agitation. The lens was extracted in SDS/urea buffer. The extraction buffer was concentrated, labeled with a fluorimetric dye, and subjected to microfluidic electrophoresis.

Results: Total strongly-adsorbed protein decreased between wear from day-one to day-twenty eight, 1.64 to 1.22 µg/lens (p=0.004, Mann-Whitney), respectively. Among the other resolved proteins lysozyme, lactoferrin, lipocalin, κ/λ Ig light chain, and monomeric IgA also exhibited statistically-significant decreases over the wear cycle (p<0.05). Most significant was the 3-fold drop in strongly-adherent monomeric IgA (p=0.000).

Conclusions: A statistically-significant decline in total strongly-adherent protein from lenses worn between day-one and day-twenty-eight occurs in parallel to a general decline in patient-reported comfort scores. This unexpected finding seems to contradict the hypothesis that strongly-adherent protein levels increase with wear time. An alternate hypothesis is that other non-protein components of the tear film (possibly cholesterol or cholesterol esters) serve to displace initially strongly-adherent protein molecules as the wear cycle progresses. A general increase in the inflammatory environment of the ocular surface may accompany the 3-fold drop in monomeric IgA. IgA is generally considered as a non- or anti-inflammatory antibody isotype and therefore may be correlated with the observed decreased in comfort over the wear period.

Keywords: 477 contact lens  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×