June 2013
Volume 54, Issue 15
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ARVO Annual Meeting Abstract  |   June 2013
Correlation between Fluorescein Leakage Maps Generated by Automated Leakage Quantification of Fluorescein Angiogram and Retinal Thickness Maps Created by OCT in Eyes with Neovascular AMD
Mostafa Hanout; Eman Magdy; Owhofasa Agbedia; Mohamed Ibrahim; Millena Bittencourt; Mohammad sadiq; Daniel Ferraz; Ahmadreza Moradi; Ahmed Fahmy; Quan Dong Nguyen
Author Affiliations & Notes
  • Mostafa Hanout
    Retinal Imaging Research and Reading Center, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD
  • Eman Magdy
    Center for Informatics Science, Nile University, Cairo, Egypt
  • Owhofasa Agbedia
    Retinal Imaging Research and Reading Center, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD
  • Mohamed Ibrahim
    Retinal Imaging Research and Reading Center, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD
  • Millena Bittencourt
    Retinal Imaging Research and Reading Center, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD
  • Mohammad sadiq
    Retinal Imaging Research and Reading Center, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD
  • Daniel Ferraz
    Retinal Imaging Research and Reading Center, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD
  • Ahmadreza Moradi
    Retinal Imaging Research and Reading Center, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD
  • Ahmed Fahmy
    Center for Informatics Science, Nile University, Cairo, Egypt
    Systems of Biomedical Engineering Department, Cairo University, Cairo, Egypt
  • Quan Dong Nguyen
    Retinal Imaging Research and Reading Center, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD
  • Footnotes
    Commercial Relationships Mostafa Hanout, None; Eman Magdy, None; Owhofasa Agbedia, None; Mohamed Ibrahim, None; Millena Bittencourt, None; Mohammad sadiq, None; Daniel Ferraz, None; Ahmadreza Moradi, None; Ahmed Fahmy, None; Quan Dong Nguyen, Genentech (F), Regeneron (F), Lux Biosciences (F), Abbott (F), GSK (F), Santen (F), Santen (C), Bausch and Lomb (C), Optos (F), Heidelberg Engineering (F)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5505. doi:
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      Mostafa Hanout, Eman Magdy, Owhofasa Agbedia, Mohamed Ibrahim, Millena Bittencourt, Mohammad sadiq, Daniel Ferraz, Ahmadreza Moradi, Ahmed Fahmy, Quan Dong Nguyen; Correlation between Fluorescein Leakage Maps Generated by Automated Leakage Quantification of Fluorescein Angiogram and Retinal Thickness Maps Created by OCT in Eyes with Neovascular AMD. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5505.

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Abstract
 
Purpose
 

To validate automated leakage quantification of fluorescein angiography (ALQFA) as an objective method to evaluate fluorescein angiographic (FA) images in patients with neovascular AMD and to determine the correlation between fluorescein leakage maps (FLMs) created by ALQFA and retinal thickness maps created by spectral domain optical coherence tomography (SD-OCT)

 
Methods
 

A minimum of two early FA frames (<1 min) and two late frames (> 2 min) as well as OCT retinal thickness maps were obtained for 20 eyes with neovascular AMD. Only images from the macular area were processed. Retinal blood vessels were identified using a matched filter technique. Vascular hyper fluorescence was suppressed to avoid misinterpretation with leakage areas. The background was modeled and subtracted from the late frame to highlight areas of fluorescein leakage. FLM was produced from the resulting difference image (DI). A color code was added to transform the grey scale on the DI to predicted retinal thickness values.

 
Results
 

Nine subfields of the FLM for each subject were compared with the corresponding subfields in the retinal thickness maps created by SD-OCT and the correlation coefficient was calculated. A significant correlation ranging from 0.702 to 0.947 was observed in 11 out of 20 eyes (55%). Reproducibility was confirmed by assessing different combinations of early and late frames for the same eye.

 
Conclusions
 

ALQFA may provide an objective and reproducible way of assessing FA sequence images, which can strengthen the importance of FA as a tool for individual neovascular AMD patient follow-up evaluations. Additional longitudinal studies are indicated to replicate the utility of ALQFA in follow up of AMD patients.

 
Keywords: 412 age-related macular degeneration • 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound)  
© 2013, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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