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Mostafa Hanout, Eman Magdy, Owhofasa Agbedia, Mohamed Ibrahim, Millena Bittencourt, Mohammad sadiq, Daniel Ferraz, Ahmadreza Moradi, Ahmed Fahmy, Quan Dong Nguyen; Correlation between Fluorescein Leakage Maps Generated by Automated Leakage Quantification of Fluorescein Angiogram and Retinal Thickness Maps Created by OCT in Eyes with Neovascular AMD. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5505.
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© ARVO (1962-2015); The Authors (2016-present)
To validate automated leakage quantification of fluorescein angiography (ALQFA) as an objective method to evaluate fluorescein angiographic (FA) images in patients with neovascular AMD and to determine the correlation between fluorescein leakage maps (FLMs) created by ALQFA and retinal thickness maps created by spectral domain optical coherence tomography (SD-OCT)
A minimum of two early FA frames (<1 min) and two late frames (> 2 min) as well as OCT retinal thickness maps were obtained for 20 eyes with neovascular AMD. Only images from the macular area were processed. Retinal blood vessels were identified using a matched filter technique. Vascular hyper fluorescence was suppressed to avoid misinterpretation with leakage areas. The background was modeled and subtracted from the late frame to highlight areas of fluorescein leakage. FLM was produced from the resulting difference image (DI). A color code was added to transform the grey scale on the DI to predicted retinal thickness values.
Nine subfields of the FLM for each subject were compared with the corresponding subfields in the retinal thickness maps created by SD-OCT and the correlation coefficient was calculated. A significant correlation ranging from 0.702 to 0.947 was observed in 11 out of 20 eyes (55%). Reproducibility was confirmed by assessing different combinations of early and late frames for the same eye.
ALQFA may provide an objective and reproducible way of assessing FA sequence images, which can strengthen the importance of FA as a tool for individual neovascular AMD patient follow-up evaluations. Additional longitudinal studies are indicated to replicate the utility of ALQFA in follow up of AMD patients.
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