June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Human graft cornea imaging with full-field optical coherence tomography
Author Affiliations & Notes
  • Wajdene Ghouali
    CHNO des Quinze-Vingts, Paris, France
  • Kate Grieve
    Institut Langevin, Paris, France
  • Otman Sandali
    CHNO des Quinze-Vingts, Paris, France
  • Elena Basli
    CHNO des Quinze-Vingts, Paris, France
  • Julien Bullet
    CHNO des Quinze-Vingts, Paris, France
  • Laurent Laroche
    CHNO des Quinze-Vingts, Paris, France
  • Vincent Borderie
    CHNO des Quinze-Vingts, Paris, France
  • Footnotes
    Commercial Relationships Wajdene Ghouali, None; Kate Grieve, None; Otman Sandali, None; Elena Basli, None; Julien Bullet, None; Laurent Laroche, None; Vincent Borderie, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 561. doi:https://doi.org/
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    • Get Citation

      Wajdene Ghouali, Kate Grieve, Otman Sandali, Elena Basli, Julien Bullet, Laurent Laroche, Vincent Borderie; Human graft cornea imaging with full-field optical coherence tomography. Invest. Ophthalmol. Vis. Sci. 2013;54(15):561. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

There is currently no efficient method for the study of epithelium and stroma of human graft corneas. The aim of our study is to evaluate the performance of a full-field optical coherence tomograhy system in the evaluation of human graft corneas.

 
Methods
 

Our study was carried out using a full-field OCT system from LLTech ®, developed for non-invasive imaging of tissue structures in depth. Images were acquired on human donor corneas (in normal and oedematous conditions) and surgical specimens of pathological corneas (Fuchs dystrophy, keratoconus, stromal keratitis)

 
Results
 

The Full-field OCT device from LLTech® enables three dimensional images to be obtained with ultrahigh resolution (1 micron in all directions) comparable to traditional histological sections. This allows a precise visualisation of the cells and the different structures (epithelium, Bowman’s membrane, stroma, Descemet’s membrane and endothelium) in normal corneas (figure 1), but also in pathological corneas (even in the presence of an oedema), with specific lesions in each condition.

 
Conclusions
 

Optical microscopy, with a detailed view of the corneal endothelium and a cell density determination, remains the « gold standard » in the study of human cornea grafts. However, full-field OCT, thanks to a more complete anatomical stydy of the cornea, could be helpful in the evaluation and the selection of human cornea grafts whose quality plays a major role in the outlook of the corneal transplant

 
 
The figure 1 shows full-field OCT images of the cornea from the 3D data stack. The upper four images are en face optical slices descending through the sample depth: EP epithelium; BM Bowman's membrane; ST stroma; DM+E Descemet's membrane and endothelium. The lower image is a depth slice through the cornea showing the different layers as labelled above. Scale bars show 100μm.
 
The figure 1 shows full-field OCT images of the cornea from the 3D data stack. The upper four images are en face optical slices descending through the sample depth: EP epithelium; BM Bowman's membrane; ST stroma; DM+E Descemet's membrane and endothelium. The lower image is a depth slice through the cornea showing the different layers as labelled above. Scale bars show 100μm.
 
Keywords: 483 cornea: storage • 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound)  
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