June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Establishment of the ocular hypertension model using the common marmoset
Author Affiliations & Notes
  • Masamitsu Shimazawa
    Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan
  • Shinsuke Nakamura
    Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan
  • Miki Miwa
    Department of Behavioral and Brain Sciences, Primate Research Institute, Kyoto University, Inuyama, Japan
  • Kazuhiro Tsuruma
    Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan
  • Makoto Aihara
    Department of Ophthalmology, University of Tokyo School of Medicine, Tokyo, Japan
  • Katsuki Nakamura
    Department of Behavioral and Brain Sciences, Primate Research Institute, Kyoto University, Inuyama, Japan
  • Hideaki Hara
    Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan
  • Footnotes
    Commercial Relationships Masamitsu Shimazawa, None; Shinsuke Nakamura, None; Miki Miwa, None; Kazuhiro Tsuruma, None; Makoto Aihara, Ono pharmaceutical company (F), Pfizer (F); Katsuki Nakamura, None; Hideaki Hara, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5649. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Masamitsu Shimazawa, Shinsuke Nakamura, Miki Miwa, Kazuhiro Tsuruma, Makoto Aihara, Katsuki Nakamura, Hideaki Hara; Establishment of the ocular hypertension model using the common marmoset. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5649. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: We established an experimental glaucoma model in the common marmoset (Callithrix jacchus).

Methods: Chronic intraocular pressure (IOP) elevation was induced by laser trabeculoplasty twice at 2-week intervals in the left eyes of 4 common marmosets. IOP was measured before and at 4, 7, 8, 11, 13 weeks after first laser treatment, and ophthalmoscopic examinations were also performed. At 13 weeks after laser treatment, each eye was enucleated, and retinal cross-sections and optic nerve were prepared for histological examination.

Results: IOP was persistently elevated to over 30 mmHg in laser-treated eyes during 13 weeks in 3 marmosets, but IOP in one marmoset was transiently increased to 26.6 mmHg at 7 weeks and then declined to normal level in the fellow eye. In ophthalmoscopy, deepened and enlarged optic disc cupping, depending on the extent of IOP elevation and duration, were observed in laser-treated eyes of 3 marmosets with persistent IOP elevation, but there was no apparent change in the optic disc in the laser-treated eye of one marmoset with transient IOP elevation. Histological examination showed marked atrophy with deepened and enlarged cupping of optic disc, thinning of retinal nerve fiber layer and retinal ganglion loss in the retina, and axonal atrophy and loss in the optic nerve, depending on the extent of IOP elevation and duration.

Conclusions: We succeeded in producing an experimental glaucoma model in the common marmoset, and this model may be useful in elucidating the pathophysiological mechanism for glaucoma.

Keywords: 627 optic disc • 568 intraocular pressure • 629 optic nerve  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×