Purchase this article with an account.
Sebastiao Cronemberger, Vinicius Oliveira, Renata Fleming, Nassim Calixto, Adalmir Dantas; Resting Potential of Rat Crystalline Lens. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5731.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To measure the resting potential (RP) in the anterior and posterior surfaces of the rat crystalline lens (CL) investigating if there is difference between the center and the four quadrants.
We performed 20 experiments (10 for each surface) on rat CL. Immediately after decapitation the eyes were removed and the posterior pole and vitreous were excised. The CL was placed in a modified Ringer solution (RS) with the cornea downwards. To measure the RP of the anterior surface of the CL the cornea was removed. The temperature in the chamber was set at 30°C by means of a thermostatic bath. RS composition in mM was Na+Cl- 100, K+Cl- 6, Ca++Cl- 2, MgSO4 1, NaHPO4 1, NaHCO3 30 and glucose 20. The membrane potential was measured using a microelectrode that was inserted inside the CL through the posterior or anterior pole at the center and at the four peripheral quadrants. The microelectrode was connected via voltmeter to a reference electrode that was immersed in RS. The signal (mV) was amplified and displayed and registered on a Grass polygraph. The external electrode is outside the CL capsule. When the microelectrode crosses the CL capsule, it sends a signal that is recorded as a negative potential in relation to the potential measured by the reference electrode. The difference between the values of the two electrodes gives the RP. The statistical analysis was made by Graph Pad Prism 6.0.
The mean, standard deviation and standard error of the RP of the rat CL are on the Table 1 below.
The RP of the center and the four quadrants of the anterior and posterior surfaces of the rat CL ranged from 40 to 58 mV with no statistically significant difference.
This PDF is available to Subscribers Only