June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Ultrastructural changes of the vitreoretinal interface during longterm follow-up after the removal of the internal limiting membrane
Author Affiliations & Notes
  • Toshio Hisatomi
    Ophthalmology, Kyushu Univ., Fukuoka, Japan
    Clinical Research Institute, Kyushu Medical Center, Fukuoka, Japan
  • Hiroshi Enaida
    Ophthalmology, Kyushu Univ., Fukuoka, Japan
  • Shoji Notomi
    Ophthalmology, Kyushu Univ., Fukuoka, Japan
  • Yukio Sassa
    Ophthalmology, Kyushu Univ., Fukuoka, Japan
  • Atsunobu Takeda
    Ophthalmology, Kyushu Univ., Fukuoka, Japan
  • Tatsuro Ishibashi
    Ophthalmology, Kyushu Univ., Fukuoka, Japan
  • Footnotes
    Commercial Relationships Toshio Hisatomi, None; Hiroshi Enaida, None; Shoji Notomi, Kyushu University (P); Yukio Sassa, None; Atsunobu Takeda, None; Tatsuro Ishibashi, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5772. doi:https://doi.org/
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      Toshio Hisatomi, Hiroshi Enaida, Shoji Notomi, Yukio Sassa, Atsunobu Takeda, Tatsuro Ishibashi; Ultrastructural changes of the vitreoretinal interface during longterm follow-up after the removal of the internal limiting membrane. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5772. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: We reported 12months ultrastructural changes after experimental vitrectomy with internal limiting membrane (ILM) peeling in the cynomolgus monkeys. To further investigate the longterm follow-up, we examined morphological changes of the vitreoretinal interface 3 years after the removal of the ILM.

Methods: In 10 primate eyes, a pars plana vitrectomy was performed followed by ICG-assisted peeling of the ILM. Morphological changes in the exposed inner surface of the retina were investigated by electronmicroscopy immediately, 3, 6 12 and 36 months after the ILM removal.

Results: The excised ILM was associated with fragments of glial tissue. The Müller cell processes were partially damaged and removed from the corresponding region of the retina. While regenerative spindle-shaped Müller cell processes developed and during 12 months, the glial cell process covered most part of the damaged retina. Notably nerve fiber layer was exposed to the vitreous space with misdirection of glial wound healing. The wound healing process developed with glial cells, however, there was no apparent ILM regeneration as a flat sheet except for deposit of basement membrane materials. The neuronal cell response against the ILM peeling was also analyzed histologically.

Conclusions: ICG assisted ILM peeling cause mild damage to the vitreoretinal interface, which was not completely restored even after 3 years.

Keywords: 688 retina • 762 vitreoretinal surgery • 540 glia  
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