June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Association of M2 macrophages in the development of fibrovascular membranes in diabetic retinopathy
Author Affiliations & Notes
  • Yoshiyuki Kobayashi
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Shigeo Yoshida
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Shintaro Nakao
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Takashi Tachibana
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Takahito Nakama
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Keijiro Ishikawa
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Yukio Sassa
    Ophthalmology, Kyushu University, Fukuoka, Japan
    Ophthalmology, Fukuoka University Chikushi Hospital, Fukuoka, Japan
  • Hiroshi Enaida
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Yuji Oshima
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Tatsuro Ishibashi
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Footnotes
    Commercial Relationships Yoshiyuki Kobayashi, None; Shigeo Yoshida, None; Shintaro Nakao, None; Takashi Tachibana, None; Takahito Nakama, None; Keijiro Ishikawa, None; Yukio Sassa, None; Hiroshi Enaida, None; Yuji Oshima, None; Tatsuro Ishibashi, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5780. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Yoshiyuki Kobayashi, Shigeo Yoshida, Shintaro Nakao, Takashi Tachibana, Takahito Nakama, Keijiro Ishikawa, Yukio Sassa, Hiroshi Enaida, Yuji Oshima, Tatsuro Ishibashi; Association of M2 macrophages in the development of fibrovascular membranes in diabetic retinopathy. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5780. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: It was recently reported that M2 macrophages play an important role in wound healing, fibrosis and cancer metastasis. However, their role in diabetic retinopathy remains elusive. The purpose of this study was to determine whether M2 macrophages can be detected in patients with proliferative diabetic retinopathy (PDR).

Methods: We measured the levels of VEGF, periostin and, CD163, a marker for M2-polarization, by sandwich enzyme linked immunosorbent assay in vitreous samples collected from patients with macular hole, epiretinal membrane, diabetic macular edema, PDR, and proliferative vitreoretinopathy (PVR). The location of CD163 and periostin was also determined by immunohistochemistry.

Results: The mean vitreous levels of CD163 were significantly higher in patients with PDR and PVR than in macular hole patients (P <0.001). There was a significant correlation between the vitreous concentrations of CD163 and periostin in patients with PDR (P <0.001). Immunohistochemical analysis showed colocalization of CD163 and periostin in fibrovascular membranes (FVMs) obtained from patients with PDR.

Conclusions: These findings indicate that M2 macrophages may be involved in the development of FVMs possibly through induction of periostin.

Keywords: 499 diabetic retinopathy • 655 proliferative vitreoretinopathy • 748 vascular endothelial growth factor  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×