June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Vitreous levels of MCP-1 and CD163 in vitreoretinal diseases
Author Affiliations & Notes
  • Takashi Tachibana
    Kyushu University, Fukuoka, Japan
  • Shigeo Yoshida
    Kyushu University, Fukuoka, Japan
  • Yoshiyuki Kobayashi
    Kyushu University, Fukuoka, Japan
  • Takahito Nakama
    Kyushu University, Fukuoka, Japan
  • Keijiro Ishikawa
    Kyushu University, Fukuoka, Japan
  • Shintaro Nakao
    Kyushu University, Fukuoka, Japan
  • Yukio Sassa
    Kyushu University, Fukuoka, Japan
  • Hiroshi Enaida
    Kyushu University, Fukuoka, Japan
  • Yuji Oshima
    Kyushu University, Fukuoka, Japan
  • Tatsuro Ishibashi
    Kyushu University, Fukuoka, Japan
  • Footnotes
    Commercial Relationships Takashi Tachibana, None; Shigeo Yoshida, None; Yoshiyuki Kobayashi, None; Takahito Nakama, None; Keijiro Ishikawa, None; Shintaro Nakao, None; Yukio Sassa, None; Hiroshi Enaida, None; Yuji Oshima, None; Tatsuro Ishibashi, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5800. doi:
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    • Get Citation

      Takashi Tachibana, Shigeo Yoshida, Yoshiyuki Kobayashi, Takahito Nakama, Keijiro Ishikawa, Shintaro Nakao, Yukio Sassa, Hiroshi Enaida, Yuji Oshima, Tatsuro Ishibashi; Vitreous levels of MCP-1 and CD163 in vitreoretinal diseases. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5800.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: MCP-1 is a chemokine associated with wound healing and fibrosis. Roles of MCP-1 and M2 macrophage in ocular disease are not fully understood. The purpose of this study was to determine the vitreous levels of MCP-1and CD163, a specific M2 macrophage marker, and the correlation of these molecules in vitreoretinal diseases.

Methods: Vitreous samples were obtained from 377 eyes of 317 patients with macular hole (MH; n = 62), diabetic macular edema (DME; n =40), proliferative diabetic retinopathy (PDR; n= 253), and proliferative vitreoretinopathy (PVR; n= 22) during pars plana vitrectomy. We also obtained vitreous samples from 53 eyes of patients with PDR who underwent secondary intraocular lens implantation approximately 6 months after the initial vitrectomy. The levels of MCP-1, CD163, and periostin in vitreous samples were measured by sandwich enzyme linked immunosorbent assay. Correlation between MCP-1, CD163, and periostin levels were calculated by Pearson correlation coefficient.

Results: The mean vitreous MCP-1 levels in patients with PDR (482.5 pg/ml) and PVR (3203 pg/ml) were significantly higher than that in patients with MH (482.5 pg/ml, P<0.001). In patients with PDR and PVR, the vitreous level of MCP-1 was correlated with that of CD163 (ρ=0.594, p<0.0001, ρ=0.7564, p=0.0003 ). The changes in the vitreous level of MCP-1 were not significant after vitrectomy.

Conclusions: Our data suggest that MCP-1 may play an important role in epiretinal fibrous proliferation, by possibly recruiting M2 macrophages.

Keywords: 654 proliferation • 557 inflammation • 762 vitreoretinal surgery  
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