Abstract
Purpose:
MCP-1 is a chemokine associated with wound healing and fibrosis. Roles of MCP-1 and M2 macrophage in ocular disease are not fully understood. The purpose of this study was to determine the vitreous levels of MCP-1and CD163, a specific M2 macrophage marker, and the correlation of these molecules in vitreoretinal diseases.
Methods:
Vitreous samples were obtained from 377 eyes of 317 patients with macular hole (MH; n = 62), diabetic macular edema (DME; n =40), proliferative diabetic retinopathy (PDR; n= 253), and proliferative vitreoretinopathy (PVR; n= 22) during pars plana vitrectomy. We also obtained vitreous samples from 53 eyes of patients with PDR who underwent secondary intraocular lens implantation approximately 6 months after the initial vitrectomy. The levels of MCP-1, CD163, and periostin in vitreous samples were measured by sandwich enzyme linked immunosorbent assay. Correlation between MCP-1, CD163, and periostin levels were calculated by Pearson correlation coefficient.
Results:
The mean vitreous MCP-1 levels in patients with PDR (482.5 pg/ml) and PVR (3203 pg/ml) were significantly higher than that in patients with MH (482.5 pg/ml, P<0.001). In patients with PDR and PVR, the vitreous level of MCP-1 was correlated with that of CD163 (ρ=0.594, p<0.0001, ρ=0.7564, p=0.0003 ). The changes in the vitreous level of MCP-1 were not significant after vitrectomy.
Conclusions:
Our data suggest that MCP-1 may play an important role in epiretinal fibrous proliferation, by possibly recruiting M2 macrophages.
Keywords: 654 proliferation •
557 inflammation •
762 vitreoretinal surgery