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TaeGi Kim, Ji Ho Yang, Jisang Han, Seung-Young Yu, Hyung-Woo Kwak; Repeatability and Reproducibility of Spectral-domain Optical Coherence Tomography in Measurement of Retinal Thickness in Rats. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5873. doi: https://doi.org/.
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To evaluate the repeatability and reproducibility of spectral-domain optical coherence tomography (SD-OCT) .
20 eyes of 10 LETO rats were anesthetized and RNFL circular scans were centered on optic nerve head with SD-OCT using built in Autorescan software. In each eyes, OCT scans were performed three times by two independent examiners. Rats were repositioned between scans. The interval between session 1 and session 2 was 1 day. The variability of total retinal thickness (TRT), thickness of retinal nerve fiber layer (RNFL) was measured. TRT and thickness of RNFL was assessed, standard deviation (SD), intraclass correlation coefficients (ICC) and coefficients of variation (CV) were calculated. Inter-examiner reproducibility was visualized by using Bland-Altman analysis.
For TRT, intrasession and intersession ICCs with SD-OCT was 0.85 or higher. CV raged from 3.9 % to 4.8%. For RNFL thickness, intra-session and inter-session ICCs with SD-OCT was higher 0.65 or higher except session I of examiner 2. Inter-examiner ICCs was 0.74 for TRT and 0.70 for RNFL. The CV was 4.4 % for TRT and 6.0% for RNFL.
Measurement of TRT and thickness of RNFL show high intra-session, inter-session and inter-examiner repeatability and reproducibility in in vivo using SD-OCT. This method will help to allowing longitudinal study designs, following disease processes over the time, and evaluation of therapeutic effects after experimental intervention.
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